纳米MnO_2与常规MnO_2粉末对Hela细胞DNA损伤的对比研究

DNA Damage of Hela Cells Induced by Nanosized MnO_2 and Normal MnO_2 Powder

为探讨纳米MnO2与常规MnO2粉末对细胞DNA损伤作用的差别,采用不同浓度的纳米MnO2与常规MnO2粉末(0、100、200、400μg·mL-1)对Hela细胞进行染毒,应用单细胞凝胶电泳(彗星实验)检测Hela细胞的损伤效应.结果表明,与对照组相比,纳米MnO2和常规MnO2各染毒组细胞尾部DNA百分率(TailDNA%)和尾矩(TailMoment)均显著增加(p<0.01);同一浓度下,纳米MnO2组细胞尾部DNA百分率和尾矩显著高于常规MnO2组(p<0.01).以上结果表明,纳米MnO2和常规MnO2粉末均能导致Hela细胞DNA损伤,且纳米MnO2的损伤作用强于常规MnO2.

In order to explore and compare the DNA damage effects on cells induced by nanosized MnO2 and normal MnO2 powder, Hela cells were exposed with different concentrations of these two powders （0, 100, 200, 400μg ·mL^-1） , The comet assay was applied to measure the DNA damage. Results showed that Tail DNA% and Tail Moment were both significantly increased in all the nanosized MnO2 and normal MnO2 powder treatment groups compared with the control. Meanwhile, at the same level, nanosized MnO2 groups induced greater damage than the normal MnO2 powder treated ones. It was concluded that both nanosized MnO2 and normal MnO2 powder could induce DNA damage to Hela cells, and nanosized MnO2 showed greater toxicity than normal MnO2 powder.