杀虫剂杀螟丹遗传毒性检测

Study on genotoxicity of pesticide cartap

目的探讨杀虫剂杀螟丹的遗传毒性。方法应用Ames试验、小鼠骨髓嗜多染红细胞微核试验、彗星试验对杀螟丹的遗传毒性进行评价。结果Ames试验中,TA97、TA98、TA100和TA102 4个标准菌株无论代谢活化或非代谢活化,皆为阴性结果;小鼠骨髓嗜多染红细胞微核试验的结果,与阴性对照组比较,差异无统计学意义;彗星试验结果显示,剂量100 mg/(kg.bw)时,尾长与溶剂对照组比较,差异有统计学意义(P<0.05),而其他评价指标与溶剂对照组比较,差异无统计学意义。结论经Ames试验、小鼠骨髓嗜多染红细胞微核试验、彗星试验,杀螟丹遗传毒性均为阴性。

Objective To study the genotoxicity of pesticide cartap. Methods The Ames test, micronucleus test and comet assay were used to detect the genotoxicity of pesticide cartap. Results With and without S9 activation, there were negative results of the TA97, TA98, TA100 and TA102 strains in Ames test. The micronucleus rates in all doses were not significantly different from the control group; In comet assay, the tail length was significantly different between the negative control and the treated groups （P 〈 0.05） in dose of 100 mg/（ kg·bw）, while there was not significant difference between the negative control and the treated groups（ P 〉 0.05 ） in other dose; The results of genetic toxicity tests were all negative, including Ames test, micronucleus test and comet assay. Conclusion The pesticide cartap has no genetic toxicity in this test.