大鼠血浆中野黄芩苷的HPLC测定及其药动学研究

Determination of Scutellarin in Rat Plasma by HPLC and Its Pharmacokinetics

建立了HPLC法测定中药复方茵山莲颗粒灌胃后大鼠血浆中野黄芩苷的浓度,并研究了其在大鼠体内的药动学。以黄芩苷为内标,采用C18柱,乙腈-四氢呋喃-0.4%磷酸(15∶35∶150)为流动相,检测波长为335nm。野黄芩苷浓度在0.025～0.16μg/ml范围内线性关系良好(r=0.9946),定量限为0.025μg/ml,日内和日间RSD均小于10%。主要药动学参数为cmax(0.765±0.158)μg/ml,tmax(3.67±0.47)h,t1/2(10.78±2.63)h,AUC0-36(7.66±1.63)μg·h·ml-1,AUC0-∞(8.46±1.79)μg·h·ml-1。

A HPLC method was established for the determination of scutellarin in rat plasma, and pharmacokinetics after ig administration of Yinshanlian granules were investigated. Baicalin was used as the internal standard. A C18 column was used, with the mobile phase of acetonitrile-tetrahydrofuran-0.4% phosphoric acid （15 ： 35 ： 150）, at the detection wavelength of 335nm. The calibration curve of scutellarin was linear in the concentration range of 0.025 - 0.16 μg/ml （r = 0.9946）. The lower limit of quantification was 0.025μg/ml. The RSDs of inter- and intra-day were less than 10%. The main pharmacokinetics parameters were as follows：cmax（0.765±0.158）μg/ml,tmax（3.67±0.47）h,t1/2（10.78±2.63）h,AUC0-36（7.66±1.63）μg·h·ml^-1,AUC0-∞（8.46±1.79）μg·h·ml^-1.