脾阴虚证大鼠模型心脏组织MAPK信号转导与调控的研究

Study on Transduction and Regulation of MAPK Signal on Rat Model of Spleen-Yin Deficiency

目的:通过观察脾阴虚证大鼠模型MAPK活性变化,探讨在脾阴虚状态下大鼠心脏组织MAPK活性变化规律及其调控机制,进一步揭示脾阴虚证的病理实质和滋脾阴法的作用,从而为脾实质的研究提供客观依据。方法:采用饮食不节、疲劳过度和药物损伤复合造模法塑造脾阴虚证动物模型。采用免疫沉淀法检测心脏组织中MAPK活性。结果:脾阴虚证模型组与正常对照组相比,心脏组织的细胞浆MAPK活性升高(P<0.05),脾阴虚证治疗组与正常对照组相比,心脏组织的细胞浆MAPK活性升高(P<0.01),与脾阴虚证模型组相比,心脏组织的细胞浆MAPK活性升高(P<0.01)。结论:脾阴虚证状态下,大鼠心脏组织MAPK参与的细胞信号转导发生改变。滋脾阴方药能有效调控脾阴虚证大鼠心脏组织MAPK活性变化,可能为滋脾阴方药作用机制之一。

Objective ： To investigate the changes of activities of mitogen-activated protein kinase （MAPK） in the heart of the rats with spleen-yin deficiency syndrome and the effective mechanism of Chinese herbs for replenishing the spleen-yin. Methods ： The rat model of spleen-yin deficiency was set up by applying the multiply factors of improper diet, overstrain and damage from abuse administration drugs. And the activities of MAPK in the heart tissue were detected by substrate phosphorylation method. Results ： （1）The MAPK activity in the heart tissue of rats with spleen - yin deficiency was increased obviously. （2）The herb of replenishing the spleen-yin could increase the MAPK activity of the cytoplasm of the heart tissue of the rats with spleen-yin deficiency. Conclusion ： It was shown that the MAPK activities of the heart tissue changed in the rats with spleen-yin deficiency syndrome, and the herbs of replenishing the spleen-yin had effect on the MAPK signal transduction pathway.