血红蛋白在DHP/PAM-CdS膜修饰金电极上的直接电化学及其与利巴韦林相互作用的研究

Direct Electrochemistry of Hemoglobin Immobilized on DHP/PAM-CdS Nanoparticles Films Modified Au Electrode and Its Interaction With Ribavirin

采用直接滴涂的方法将血红蛋白固定到双十六烷基磷酸酯(DHP)/聚丙烯酰胺包裹的硫化镉纳米粒子(PAM-CdS)膜修饰金电极的表面,通过循环伏安法研究了血红蛋白在修饰金电极上的直接电化学行为.在0.1MpH=6.0的磷酸缓冲溶液中,该修饰电极有一对氧化还原峰,其式电位为0.0405V(以饱和甘汞电极为参比电极),并且峰电位随着溶液pH值的增加而负移,其斜率为-40.1mV.pH-1,表明此反应过程是一电子一质子过程.利用此修饰电极,我们研究了血红蛋白与抗病毒药物利巴韦林间的相互作用.

Direct electrochemistry of hemoglobin （Hb） immobilized on dihexadecylphosphate （DHP）/ polyacrylamide-capped cadmium sulfide nanoparticles （PAM-CdS） film modified Au electrode was investigated. The immobilized Hb displayed a pair of well-defined redox peaks with a formal potential of 0. 0405 V （versus saturated calomel electrode） in the pH 6.0 phosphate buffer solution （PBS）. Peak potential was shifted linearly in the negative directional with increasing solution pH from 5.0 - 9.0 （with a slope of - 40.1 mV per pH unit）. Electron transfer between Hb and Au electrode was greatly facilitated in the DHP/PAM-CdS films microenvironment. The interaction between Hb and ribavirin was investigated by electrochemical technique. The peak current is proportional to the concentration of ribavirin over the range of 2.80 × 10^-5 to 1.10 × 10^-4 mol/L, the linear regression equation was I （μA） = 3. 7797- 0. 04251c（μM） with a correlation coefficient r = 0. 9962, and the detection limit was 9.20× 10^-6mol/L （S/N= 3）.