NSE和ERα在老年人和AD病人大脑海马神经元中共表达(英文)

Colocalization of NSE and ERα in the Hippocampus of Elderly People and Alzheimer's Disease

服用雌激素可以防止或延缓绝经后妇女患Alzhei mer病.神经元特异性烯醇化酶(NSE)为神经元特异性标记物.运用荧光免疫技术,观察了NSE和雌激素受体α(ERα)在老年人和AD病人大脑海马神经元中共存的分布.结果显示,在对照组和AD组NSE免疫阳性神经元均主要分布海马DG区的门区和CA4区.一些AD病人海马CA1、CA2和/或CA3没有观察到NSE免疫阳性反应.NSE免疫着色主要位于胞质和突起,部分区域也弥散于胞间质.ERα免疫反应广泛分布在老年人和AD病人海马神经元,且主要位于胞质,少量位于胞核.NSE和ERα免疫双标记主要分布在对照组海马门区和CA4区,而在AD组较少;且多着色于胞质.在老年组,多数NSE阳性神经元与ERα共着色,但仅有部分ERα阳性神经元NSE也着色.结果提示,雌激素可能通过其受体介导对神经元起保护作用的靶细胞仅为NSE和ERα双着色的那部分神经细胞.

Postmenopausal estrogen use may decrease the risk, and delay the onset and progression, of Alzheimer＇s disease （AD）. Neuron Specific Enolase （NSE） is a major glycolytic pathway enzyme found in all neurons. By means of fluorescence immunocytochemistry, the present study investigated the colocalization of NSE and estrogen receptor α （ERα） in the human hippocanpus in controls and in AD cases. The results showed that, in both controls and AD cases, NSE immunoreactive neurons were mainly located in the hilus of the dentate gyrus and in the subfield CA4 of hippocampus. In some AID patients, NSE-positive neurons were not detected in subfields CA1, CA2, and/or CAB. The NSE immunoreactivity was primarily observed in the cytoplasm and processes but also in the mesenchyrnal both in controls and in AD cases. ERα immunoreactivity was widely distributed in neurons, mainly concentrated in the cytoplasm, but some nuclei were also positive. The double labeled neurons for NSE and ERα, observed mainly in the elderly people but little in the AD patients, were primarily distributed in the dentate gyrus and the subfield CA4 of hippocampus. Double labeling of NSE and ERα was mainly localized in cytoplasm. Most NSE-positive neurons were immunocytochemically stained for ERα, but only a subset of ERα-positive neurons was immunocytochemically stained for NSE in the hippoeampus of elderly human beings. Our present findings indicate a possible mechanism that estrogens play the protective effects on neurons through the mediation of ERα only in the NSE- immunoreaetive nerve cells.