解毒化瘀方对内毒素血症大鼠肝细胞线粒体ATPase 6、ATPase 8基因表达的影响

Effect of Jiedu Huayu Fang(JDHYF) on hepatocyte mitochondrial ATPase 6,ATPase 8 gene expression in endotoxemia rats

目的:观察解毒化瘀方对内毒素血症大鼠肝细胞线粒体ATPase 6、ATPase 8基因表达的影响。方法:将大鼠分成正常对照、内毒素6、12、24小时和解毒化瘀方6、12、24小时组,检测肝线粒体Na+K+-ATP、Ca+Mg+-ATP酶活性和ATPase 6、ATPase 8 mRNA的表达。结果:内毒素各组Na+K+-ATP、Ca+Mg+-ATP酶活性和ATPase 6、ATPase 8 mRNA表达与正常组比明显下降;与对应的内毒素组比,解毒化瘀方12、24小时组Na+K+-ATP、Ca+Mg+-ATP酶活性显著增加,24小时组ATPase 6、ATPase 8表达显著提高。结论:内毒素能造成大鼠肝线粒体ATPase 6、ATPase 8基因表达下降,解毒化瘀方能干预内毒素血症大鼠ATPase 6、ATPase 8基因表达下调,具有保护肝细胞的作用。

Objective： To observe the effect of JDHYF on hepatocyte mitochondrial ATPase 6, ATPase 8 gene expression in endotoxemia rats. Methods： Fifty six rats were divided into normal control group, endotoxin 6, 12, 24 hours groups and JDHYF6, 12, 24 hours group, then the activities of Na^＋K^＋-ATPase, Ca^＋Mg^＋-ATPase and expression of ATPase6, ATPase 8 mRNA in hepatocyte mitochondrial were measured. Results： Comparing normal control group, the activities of Na^＋K^＋-ATPase, Ca^＋Mg^＋-ATPase and expression of ATPase 6, ATPase 8 mRNA were obviously reduced in endotoxin 6, 12, 24 hours groups; Comparing respective endotoxin groups, the activities of Na^＋K^＋-ATPase, Ca^＋Mg^＋-ATPase were gone up obviously in JDHYF 12, 24 hours groups, the expression of ATPase 6, ATPase 8 mRNA was gone up obviously in JDHYF 24 hours groups. Conclusion： Endotoxin can cause the expression of hepatocyte mitochondrial ATPase 6, ATPase 8 mRNA to descend in rats, the JDHYF can effectively repress the descent of ATPase 6, ATPase 8 mRNA gene expression in endotoxemia rats, and protect the liver cell.