固相萃取高效液相色谱测定鱼腥草、甜茶中的糖

Study on sugars in Lithhocarpus polgstachrch Rehd and Houttuynia solid phase extraction and RP-HPLC

研究了高效液相色谱法测定鱼腥草和甜茶中的糖.样品中的糖提取液用Waters Sep-pak-C18固相萃取小柱预分离,以Waters carbohydrate高效糖柱为固定相,乙腈-水(体积比70∶30)为流动相分离,ELSD2000蒸发光散射检测器检测,实现了8种水溶性单糖和二糖的同时测定.

Determination of sugars in Lithhocarpus polgstachrch Rehd and Houttuynia with HPLC and include the contents of water soluble sugars and the component of polysaccharides. The water soluble sugars chromatography were extracted with water by ultrasonic oscillation and by solid phase extraction with Sep-pak C18 cartridge. Sugars were separated on a Waters high performance carbohydrate column as solid phase, with V （acetonitrile） ： V （water） = 70：30 as mobile phase, and detected with evaporative light-scatter detector. The method was applied to the determination of water soluble monosaccharides and polysaccharides at the same time.