胆道闭锁肝脏组织MMP-2、TGF-β_1的表达及意义

The expression of MMP-2,TGF-β_1 in liver of biliary atresia and their relations with hepatic fibrosis.

目的研究胆道闭锁患儿肝脏组织中MMP-2、TGF-β1的表达,探讨其与胆道闭锁患儿肝纤维化的关系。方法利用免疫组化法(SP法)检测33例胆道闭锁患儿肝脏组织中MMP-2、TGF-β1蛋白的表达,通过图像分析技术进行定量研究。结果胆道闭锁患儿肝组织中MMP-2、TGF-β1蛋白均有不同程度的表达,以肝细胞胞浆、胞膜表达最为明显。MMP-2、TGF-β1蛋白在肝组织中的阳性表达随肝纤维化程度的加重而增强,呈正相关(前者r=0.5063,P<0.05;后者r=0.6179,P<0.05)。MMP-2、TGF-β1在胆道闭锁患儿肝组织中的表达强度明显高于对照组(P<0.01),MMP-2与TGF-β1的表达有高度相关性(r=0.9200,P<0.01)。结论MMP-2、TGF-β1与胆道闭锁肝纤维化的发生、发展密切相关;抑制MMP-2、TGF-β1的表达有望阻止胆道闭锁患儿肝纤维化进程。

Objective To study the expression of MMP-2, TGF-β1 in biliary atresia （BA）liver tissue and their relations with hepatic fibrosis. Methods The immunohistochemical method （SP） was used to detect expression of the protein of MMP-2, TGF-β1 in liver tissues of thirty -three biliary atresias. The positive expression was quantitatively analyzed by image analysis technology. Results There were different degree expressions of MMP-2, TGF-β1 protein in hepatic tissue of BA. Subcellular locations of expression of these protein were mainly cytoplasma and membrane. Positive expressions of MMP-2, TGF-β1 were increased with progress of hepatic fibrosis degree （the former r = 0.5063, p 〈 0.01, the latter , r = 0.6179, p 〈 0.05）. Expression degree of MMP-2 and TGF-β1 protein in hepatic tissue of children with BA was significant high than that of in control group （p 〈 0.01）. The expression of MMP-2 was obviously correlated to expression of TGF-β1 in liver tissue of children with BA （r = 0.9200,P 〈 0.01）. Conclusions The levels of MMP-2,TGF are closely related to occur and development of hepatic fibrosis in biliary atresia. So inhibiting the expression of MMP-2 and TGF-β1 could prevent the progress of hepatic fibrosis for children with biliary atresia.