摘要
目的探讨人成骨细胞(MG-63)与人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVECs)共同培养后,对成骨细胞功能的影响。方法取MG-63与人HUVECs直接联合培养于培养皿中,通过对人成骨细胞内碱性磷酸酶(ALP)活性和骨钙素(OC)定量测定,观察HUVECs对人成骨细胞功能的影响。结果人成骨细胞与HUVECs直接联合共培养后,两种细胞均生长良好,人成骨细胞内ALP活性和OC定量测定结果,共培养组明显高于对照组。结论HUVECs在体外与人成骨细胞直接联合共培养中,有促进人成骨细胞活性的作用。
Objective To observe the influence of human osteoblast（MG-63 cells） function after directly co-cultured with human umbilical vein endothelial cells（HUVECs） in vitro. Methods MG-63 ceils and HUVECs were co-cultured directly in vitro. The function of human osteoblasts was assessed by alkaline phosphatase（AlP）activity and osteocalcin（OC） assay. Results Both MG-63 cells and HUVECs could grow well after co-cultured directly. The AlP activity and OC content of MG-63 cells increased significantly. Conclusion HUVECs can increase the biological activity of human osteoblast when co-cultured directly with it.
出处
《口腔医学》
CAS
2007年第6期284-286,301,共4页
Stomatology
基金
辽宁省教育厅资助项目(05L481)
关键词
人成骨细胞
人脐静脉内皮细胞
碱性磷酸酶
骨钙素
human osteoblast
human umbilical vein endothelial cells
alkaline phosphatase
osteocalcin
