微量元素硒对宁夏枸杞抗氧化系统的影响

Influence of microelement selenium on the antioxidant system of lycium barbarum

目的研究微量元素硒对宁夏枸杞抗氧化系统的影响。方法将30株幼苗随机分为对照组、亚硒酸钠(Na2SeO3)0.5 g.kg-1组和2.0 g.kg-1组,每组10株。观察不同浓度的Na2SeO3对枸杞谷胱甘肽过氧化物酶(GSH-Px)、超氧化物岐化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性和谷胱甘肽(GSH)、抗坏血酸(ASC)以及丙二醛(MDA)含量的影响。结果Na2SeO30.5 g.kg-1组GSH-P活性、GSH含量、ASC含量和SOD活性明显高于对照组(P<0.05),而MDA含量、CTA及POD活性与对照组比较无显著差异(P>0.05);Na2SeO32.0 g.kg-1组GSH-P活性、GSH含量、ASC含量、SOD活性、CTA活性及POD活性明显高于对照组(P<0.05,P<0.01),MDA含量明显低于对照组(P<0.05)。结论Na2SeO3能够提高宁夏枸杞中抗氧化酶的活性和抗氧化剂的含量,降低枸杞中MDA的含量,从而增强宁夏枸杞的抗氧化作用。

Objective To study the effects of microelement selenium on the antioxidant system of Lycium barbarum, Methods Thirty tender seedlings were randomly divided into control group, sodium selenite（ Na2 SeO3 ） 0.5 g · kg^-1 group and 2.0 g· kg^-1 group（n = 10）. The effects of different concentration of Na2SeO3 on the activities of glutathione peroxidase （GSH- Px）, superoxide dismutase （ SOD ）, catalase （ CAT ）, peroxidase （ POD ） and the contents of glutathione （ GSH ）, ascorbate （ASC） ,malondiaidehyde （MDA） of Lycium barbarum were studied. Results The activities of GSH-Px,SOD and the contents of ASC, GSH in Na2SeO3 0.5 g·kg^-1 group were higher than these in control group （P 〈 0.05 ） , but the contents of MDA and the activities of CAT,POD were indistinctive between two group. The activities of GSH-Px, SOD, CTA, POD and the contents of ASC, GSH in Na2 SeO3 2.0 g· kg^-1 group were higher than these in control group （ P 〈 0.05, P 〈 0. 01 ） , and the contents of MDA was lower（ P 〈 0.05 ）. Conclusion Na2 SeO3 can increase the activities of antioxidant enzymes and the contents of antioxidant, and decrease the content of MDA, so it can enhance the antioxidant function of Lyeium barbarum.