恩诺沙星在菲律宾蛤仔体内的消除规律研究

Study on elimination of enrofloxacin in Ruditapes philippinarum

以5mg/L恩诺沙星浸浴菲律宾蛤仔(Ruditapes philippinarum)24h,取血液、鳃、内脏团、足、闭壳肌5种组织,反相高效液相色谱法测定其中的药物浓度。结果表明,恩诺沙星按一级动力学过程从体内消除,在5种组织中消除速率快慢不一,其在菲律宾蛤仔血液、鳃、内脏团、足、闭壳肌中的消除曲线方程分别为c=6.995e-0.0853t,c=2.7957e-0.1177t,c=1.539e-0.1013t,c=1.7015e-0.1535t,c=2.010e-0.1414t,消除半衰期T1/2分别为6.284,5.889,6.483,4.516,3.679h。恩诺沙星在水产品中的最大残留限量为50μg/kg,在本实验条件下,建议临床休药期不小于2d。

The present preclinical study was performed Ruditapes philippinarum after bath treatment of 5 mg/L for to investigate the execration of enrofloxacin （EF） in 24 h. Plasma and tissue concentrations of EF were determined by HPLC. The equations of elimination curve of enrofloxacin in plasma, gill, visceral mass, foot and adductor muscle were c=6.995e^-0.0853t,c=2.7957e^-0.1177t,c=1.539e^-0.1013t,c=1.7015e^-0.1535t,c=2.010e^-0.1414t, respectively. The elimination of half-lives （T1/2） was 6.284, 5.889, 6.483, 4.516, 3.679 h, respectively.