2种消毒剂对HBsAg、HBV DNA灭活效果的试验观察

EXPERIMENTAL OBSERVATION ON DESTRUCTIVE EFFECTS OF TWO KINDS OF DISINFECTANTS ON HBsAg AND HBV DNA

[目的]对复合季铵盐消毒剂与2%强化戊二醛破坏HBsAg及HBVDNA的效果进行对比观察。[方法]采用悬液定量法进行试验。[结果]在19℃～21℃,以含有效季铵盐成分5000mg/L该消毒剂水溶液对HBsAg悬液作用30min,6000mg/L时作用20min,10000mg/L时作用10min,可有效地破坏乙肝表面抗原的抗原性;以含有效季铵盐成分5000mg/L该消毒剂水溶液对含HBVDNA的血清作用30min,含有效季铵盐成分6000mg/L、10000mg/L分别作用10min,对HBVDNA的杀灭率达99.9%。2%强化戊二醛与HBsAg悬液作用10min,可有效地破坏乙肝表面抗原的抗原性,与含HBVDNA的血清作用10min,对HBVDNA的杀灭率达99.9%。[结论]复合季铵盐消毒剂与2%强化戊二醛均可将HBsAg及HBVDNA破坏,在消毒实践中可选择使用。

[Objective] To observe the destructive effects of compound quaternmary ammonium salt disinfectant and 2% potentate glutaraldehyde on HBsAg and HBV DNA, [ Methods] Suspension quantitative test was carried out. [ Results] At 19℃-21℃, the solution containing 5 000 mg/L compound quaternary ammonium salt with a 30 min contact with HBsAg suspension, the solution containing 6 000 mg/L quaternary ammonium salt with a 20 rain contact and 10 000 mg/L with a 10 rain contact, could all destroy the antigenicity of HBsAg. For the solution containing 5 000 mg/L compound ammonium sail with a 30 min contact with HBV DNA serum, containing 6 000 mg/L, 10 000 mg/L compound quaternary ammonium salt with 10 rain contact, the kill rate of HBV DNA was 99.9%. 2% potentate glutaraldehyde with a 10 min respective contact with HBsAg suspension and HBV DNA serum, could effectively destroy the antigenicity of HBsAg and get the kill rate of HBV DNA to be 99.9%. [Conclusion] Both compound quaternary ammonium salt disinfectant and 2% potentiated glutaraldehyde can destroy the HBsAg and HBV DNA, which can be used in the disinfection practices.