摘要
背景与目的:研究表明,极化调节蛋白非典型蛋白激酶C(atypical protein kinase C,aPKC)亚型aPKC-ι和E-cadherin在一些恶性肿瘤中异常表达对肿瘤的发生、发展起着重要作用。本研究分析aPKC-ι和E-cadherin在胆管癌组织中的表达与临床病理的关系,探讨胆管癌侵袭转移的分子机制。方法:采用免疫组化EnVision法检测9例非癌胆管疾病组织及35例胆管癌组织中aPKC-ι和E-cadherin表达,分析两者与胆管癌临床病理特征、侵袭性的关系。结果:aPKC-ι在胆管癌组织中的阳性高表达率为68.6%明显高于非癌胆管疾病组织的11.1%(P=0.006),E-cadherin在胆管癌中的高表达率为37.1%明显低于非癌胆管疾病组织的88.9%(P=0.016),且两者间呈负相关(r=-0.287,P<0.05);胆管癌组织分化程度、侵袭性与aPKC-ι表达呈正相关(P<0.05),与E-cadherin表达呈负相关(P<0.05)。结论:aPKC-ι和E-cadherin的表达与胆管癌分化程度和侵袭性相关,aPKC-ι可能作为一种极化调节相关蛋白在胆管癌侵袭、转移中发挥作用。
BACKGROUND ＆ OBJECTIVE. Studies showed that the abnormal expression of atypical protein kinase C t subtype （aPKC-ι） and E-cadherin plays an important role in tumor genesis and progression. This study was to investigate the expression of aPKC-ι and E-cadherin in cholangiocarcinoma, and analyze molecular mechanisms of the invasion and metastasis of cholangiocarcinoma. METHODS, The expression of aPKC-ι and E-cadherin in 9 specimens of benign bile duct tissues and 35 specimens of cholangiocarcinoma was detected by EnVision immunohistochemistry, and their correlations to the clinicopathologic characteristics and invasion of cholangiocarcinoma were analyzed. RESULTS, The positive rate of aPKC-ι was significantly higher in cholangiocarcinoma than in benign bile duct tissues （68.6% vs. 11.1%, P= 0.006）, while the positive rate of E-cadherin was significantly lower in cholangiocarcinoma than in benign bile duct tissues （37.1% vs. 88.9%, P= 0.016）. aPKC-ι expression was negatively correlated to E-cadherin expression （r= -0.287, P〈 0.05）. aPKC-ι expression was positively and E-cadherin expression was negatively correlated to the differentiation and invasion of cholangiocarcinoma （P〈 0.05）. CONCLUSIONS. The expression of aPKC-ι and E-cadherin may reflect the differentiation and invasive potential of cholangiocarcinoma. As a polar regulation-associated protein, aPKC-ι may play a role in the invasion and metastasis of cholangiocarcinoma.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2007年第7期715-718,共4页
Chinese Journal of Cancer
