卵圆细胞在实验性肝癌发生过程中的演变特征

Evolutive Characters of Oval Cells in Experimental Hepatocarcinogenesis

背景与目的:卵圆细胞在肝癌发生过程中的作用至今还不十分明了。本研究拟通过动态的方法观察卵圆细胞在肝癌发生过程中的演变规律,揭示卵圆细胞与肝癌发生之间的关系。方法:构建实验性肝癌的大鼠诱癌模型,运用常规HE染色、免疫组织化学和爱新蓝特殊染色等方法,动态观察卵圆细胞在肝癌发生过程中的演变规律。结果:HE和免疫组化结果显示,在诱癌的第4周即可见散在的卵圆细胞在门管区附近出现,卵圆细胞OV-6免疫染色呈阳性。在诱癌的第8周和第14周,OV-6阳性细胞逐渐增多,并向肝小叶实质内深入,将肝组织分割成假小叶状。到诱癌的第17周和第24周,多个癌灶出现,同时OV-6阳性细胞的总体数量下降,癌灶内可观察到OV-6阳性细胞。爱新蓝特殊染色显示,诱发的肿瘤属混合性肝癌,其中胆管上皮细胞癌爱新蓝染色呈阳性,肝细胞癌染色呈阴性。结论:卵圆细胞在肝癌的发生过程中可能扮演重要的角色。

BACKGROUND ＆ OBJECTIVE： The role of oval cells in hepatocarcinogenesis is unclear yet. This study was to explore the correlation of oval cells to hepatocarcinoma through dynamic observation on evolutive characters of oval cells in experimental hepatocarcinogenesis. METHODS： Male SD rats were fed with 3＇-me-DAB to establish an animal model of experimental hepatocarcinoma. Evolutive characters of oval cells in liver tissue during experimental hepatocarcinogenesis was dynamically observed with routine HE staining, Alcian blue staining, and immunohistochemistry. RESULTS： Oval cells （OV-6-positive） appeared sparsely around the portal tract in the 4th week of tumor-induction. In the 8th and the 14th weeks, OV-6-positive cells were increased gradually and expanded into hepatic Iobules; the hepatic tissue was divided as pseudo-lobule-like. Till the 17th and the 24th weeks, carcinoma foci were formed, meanwhile, the total amount of oval cells were decreased, and OV-6-positive cells were observed in carcinoma foci. On Alcian blue-stained preparations, two distinct histologocal types of carcinoma foci could be seen： cholangioepithelial carcinoma foci were positive and hepatocellular carcinoma foci were negative. CONCLUSION： Oval cells, as intrahepatic stem cells, might play an important role in hepatocarcinogenesis.