喉癌前病变气滞血瘀证与VEGF的相关性研究

Correlation of Vascular Endothelial Growth Factor with Precancerous Laryngeal Lesions Differentiated as Qi Stagnation and Blood Stasis

目的:探讨气滞血瘀证喉癌前病变与血管內皮生长因子(VEGF)表达、喉黏膜恶变趋势的相关性。方法:采用免疫组化法检测,将符合气滞血瘀证喉癌前病变诊断标准患者设为试验组(35例),同时以痰浊凝聚型声带息肉患者作对照组(35例),另外取病理科存档的喉癌病理组织标本作为喉癌组(20例),进行VEGF检测。结果:试验组及喉癌组VEGF阳性表达与对照组比较,差异均有非常显著性意义(P<0．01),3组喉组织的VEGF表达阳性强度分级有程度上的差别,其表达强度为喉癌组(喉癌)>试验组(喉癌前病变)>对照组(声带息肉)。结论:喉癌前病变气滞血瘀证与VEGF的表达存在相关性,喉癌前病变中气滞血瘀证的悉变趋势高于痰浊凝聚证。

Objective: To observe the correlation of expression of vascular en-dothelial growth factor (VECF) with the malignant changes of laryngeal mucosa in pre-cancerous laryngeal lesions (PLL) patients with Qi stagnation and blood stasis. Methods: Immunohistochemical method waa used to detect VECF expression in PLL patients with Qi stagnation and blood stasis ( n =35), in vocal cord polyp patients with accumulation of phlegm turbidity ( n = 35) and in laryngeal carcinoma pathologic specimen obtained from the filed records in pathological department (n =20). Results: The VECF expression in PLL and laryngeal carcinoma patients was positive, the difference being significant as compared with that in cord polyp patients ( P < 0.01). The degree of VECF positive expression in laryngeal carcinoma patients, in PLL patients and in vocal cord polyp patients was in a decreasing sequence. Conclusion: There exists a correlation between VECF expression and PLL with Qi stagnation and blood stasis, and the trend of malignant changes in PLL patients with Qi stagnation and blood stasis is obvious as compared with that in vocal cord polyp patients with accumulation of phlegm turbidity.