甘露醇对大鼠小肠移植缺血再灌注损伤的保护作用

Protective effects of mannitol against small bowel ischemia and reperfusion injury after transplantation in rats

目的:探讨甘露醇对大鼠小肠移植缺血再灌注(ischemia and reperfusion,I/R)损伤的保护作用及其作用机制.方法:建立大鼠小肠移植I/R损伤模型.雄性SD大鼠随机分为假手术组、I/R组和甘露醇处理组.对I/R损伤后不同时间(0.5,1,1.5 h)的小肠标本进行组织病理学观察并检测小肠组织中超氧化物歧化酶(SOD,U/mgprot)和丙二醛(MDA,nmol/mgprot)的含量.结果:不同时间点I/R组小肠绒毛高度和黏膜厚度低于假手术组(P<0.01)和甘露醇处理组(P<0.05).不同时间点I/R组(16.24±4.56,18.50±2.89.19.42±2.21)MDA水平高于假手术组(10.26±1.72,P<0.01)和甘露醇处理组(11.24±1.51,14.03±3.12,16.06±3.62;P<0.05);而SOD水平低于假手术组和甘露醇处理组(398.36±18.81,363.16±16.29,325.66±14.58 vs 447.97±16.94;422.30±17.41,385.09±19.11,346.15±14.10;P<0.01或P<0.05).结论:甘露醇可通过清除氧自由基的方式对移植小肠缺血再灌注损伤起到保护作用.

AIM： To investigate the effect of mannitol on small bowel ischemia and reperfusion （I/R） injury after transplantation and its possible mechanism. METHODS： Rat models of jury after transplantation small bowel I/R in- were established in male rats. All the rats were randomly divided into sham operation group, I/R group and mannitol-treated group. After 0.5, 1 and 1.5 hours of I/R, intestinal tissues were collected for histopathological examination. The content of rnalondialdehyde （MDA） and activity of superoxide dismutase （SOD） in the intestinal tissues. RESULTS： The height and thickness of intestinal intestinal villi were significantly lower in I/R group than those in sham operation group （P 〈 0.01） and mannitol-treated group （P 〈 0.05）. At different time points, the content of MDA was markedly higher in I/R group （16.24 ± 4.56, 18.50 ± 2.89, 19.42 ± 2.21） than that in sham operation group （10.26 ± 1.72, P 〈 0.01） and mannitoltreated group （11.24 ± 1.51, 14.03 ± 3.12, 16.06 ±3.62; P 〈 0.05）, but the activity of SOD was nota- bly higher （398.36 ±1 8.81, 363.16 ± 16.29, 325.66 ±1 4.58 vs 447.97 ± 16.94; 422.30 ±1 7.41, 385.09 ±19.11, 346.15 ± 14.10; P 〈 0.01 or P 〈 0.05）. CONCLUSION： Mannitol has protective effect against small bowel I/R injury after transplantation in rats by eliminating free radicals.