胃癌组织中MMP-2和E—cadherin的表达及意义

Expressions and significance of MMP-2 and E-cadherin in gastric cancer tissues

目的检测胃癌组织中基质金属蛋白酶-2（MMP-2）和E-钙黏附素（E-cadherin）的表达，探讨其与胃癌侵袭转移的关系。方法对我科2003年12月至2004年4月30例胃癌手术患者，采用免疫组化染色检测胃癌组织中MMP-2和E-cadherin的表达情况。结果70％（21／30）的胃癌组织中MMP-2表达阳性。MMP-2表达阳性与浸润深度（P=0．022）、淋巴结转移（P=0．030）和肿瘤分化程度（P=0．043）密切相关，但与性别、年龄、肿瘤位置和直径、Lauren分型及淋巴管侵袭无关（P〉0．05）。46．7％（14／30）的胃癌组织中E-cadherin表达阴性。E-cadherin表达阴性与Lauren分型（P=0．026）、肿瘤分化程度（P=0．011）、淋巴结转移（P=0．038）和淋巴管侵袭（P：0．001）密切相关。结论胃癌组织中MMP-2表达上调和E-cadherin表达下移可能参与肿瘤侵袭转移的发生。

Objective To detect expressions of matrix metalloproteinase-2 （MMP-2 and E-cadherin in gastric cancer tissues and evaluate their relationship with tumor invasion and metastases. Methods Immunochemical technique （EliVisionTM plus） was used to detect expressions of MMP-2 and E-cadherin in tumor tissues from 30 cases with gastric carcinoma admitted into our hospital from December 2003 to April 2004. Results Expression of MMP-2 was positive in 21 specimens （70%） and negative in 9 （30%）. Positive expression of MMP-2 closely correlated with infiltration depth （ P =0.022）, lymph node metastasis （ P =0.030） and tumor differentiation （ P =0.043） but had none relation with age, gender, tumor location, tumor diameter, Lauren classification and lymphatic invasion （ P 〉 0.05）. Of 30 specimens, 14 （46.7%） had negative expression of E-cadherin, which was significantly associated with the Lauren classification （ P = 0. 026 ） , lymph node metastasis （ P -- 0.011 ）, differentiation grade （ P =0.004） and lymphatic invasion （ P =0.001 ）. ConclusionPositive expression of MMP-2 and negative expression of E-cadherin may contribute to development of tumor invasion and metastases of gastric carcinoma.