摘要
AIM: To evaluate the receptor protein which can specifically bind to β2GPⅠon the membrane of hepatocellular carcinoma (HCC) cell line SMMC-7721, and to study the biological function of the receptor.METHODS: Through β2GPⅠ-affinity chromatography column, the peptid-polysome-mRNA complex, which can specially bind to β2GPⅠ, stayed with the column and was separated from the whole polysome of liver cells, and then eluted and collected. Using cDNA synthesis kit and cDNA PCR kit, the corresponding cDNA was obtained and sequenced. RT-PCR was used to amplify annexinⅡ, and flow cytometry was used to study the competitive binding of annexinⅡ with β2GPⅠto SMMC-7721.RESULTS: A total of 1.1 kb of the cDNA fragment of the specific binding protein of β2GPⅠon liver cell membrane was obtained. The sequence of cDNA shared high homology with human annexinⅡ (98%). AnnexinⅡ was expressed on the membrane of SMMC-7721, and could compete with β2GPⅠfor combining with SMMC-7721.CONCLUSION: The receptor for β2GPⅠon membrane of SMMC-7721 cells is annexinⅡ, which might bridge HBV to infect hepatocytes.
AIM： To evaluate the receptor protein which can specifically bind to β2GP Ⅰ on the membrane of hepatocellular carcinoma （HCC） cell line SMMC-7721, and to study the biological function of the receptor. METHODS： Through β2GP Ⅰ -affinity chromatography column, the peptid-polysome-mRNA complex, which can specially bind to β2GPⅠ, stayed with the column and was separated from the whole polysome of liver cells, and then eluted and collected. Using cDNA synthesis kit and cDNA PCR kit, the corresponding cDNA was obtained and sequenced. RT-PCR was used to amplify annexin Ⅱ, and flow cytometry was used to study the competitive binding of annexin Ⅱ with β2GPⅠ to SMMC-7721. RESULTS： A total of 1.1 kb of the cDNA fragment of the specific binding protein of β2GPⅠ on liver cell membrane was obtained. The sequence of cDNA shared high homology with human annexin Ⅱ（98%）. Annexin Ⅱ was expressed on the membrane of SMMC-7721, and could compete with β2GP Ⅰ for combining with SMMC-7721. CONCLUSION： The receptor for β2GP Ⅰ on membrane of SMMC-7721 cells is annexinⅡ, which might bridge HBV to infect hepatocytes.
基金
Supported by The National Nature Science Foundation of China,No. 30070338
