盖诺顺铂化疗加放疗对乳腺癌MCF-7细胞作用时机的实验研究

Timing of radiotherapy with NVB plus DDP chemotherapy on breast cancer cells MCF-7

目的放化同期综合治疗应用越来越广泛,主要机制是放化疗对肿瘤组织的协同杀灭作用。该研究对乳腺癌放化同期治疗有效性、时机选择进行实验探讨。方法选用盖诺顺铂联合化疗(NP方案)对乳腺癌MCF-7细胞放化同期放射和联合化疗不同间隔时间后放射,比较单纯照射组、同期化放疗组和联合化疗后不同间隔时间放射组的细胞存活率(Survival Fraction,SF)。结果联合化疗加同期放射后SF较单独放射更低。比较联合化学药物处理后不同间隔时间放射,发现SF值最低分别是化疗处理后间隔4h,12h和36h组,联合化疗加同期放射组SF值居中,而间隔48h和72h后照射组,SF值明显上升,SF最低与最高值相差约15倍。结论联合化放疗对乳腺癌MCF-7细胞具有较强的协同杀灭作用,化放联合治疗的时机选择在肿瘤细胞杀灭过程中具有重要的意义,间隔48h和72h后照射放化疗协同杀灭效应减弱。

Aim and Background General treatment concurrent radiotherapy and and more widely. The main mechanism is killing action from combined roles of chemotherapy has been applied more chemotherapy and radiotherapy. The effect of concurrent radiochemotherapy and timing has been investigated on MCF-7 cells in this paper. Method The results of survival fractions （SF） among radiotherapy alone, combinations of vinorelbine （NVB） and cisplatin （DDP） chemotherapy with various interval radiations had been compared. Result SF of group combined chemotherapy with irradiation was much lower than that of group radiation alone. The SFs of MCF-7 cells by irradiated at 4h, 12h and 36h after chemotherapy were the lowest. The SF at Oh is middling. The SFs at 48h and 72h after chemotherapy had been found rising greatly. The highest SF was about 15 frees of the lowest. Conclusion Combination of concurrent chemotherapy with irradiation can make much stronger combined killing action on MCF-7 cell. Timing of the combination takes great significance during this process. The combined killing effect decreases greatly at 48h and 72h of the interval after chemotherapy.