柞蚕雄蛾液对酒精性脂肪肝小鼠保护作用的研究

The experiment of male China oak silkworm liquid's obviating function on alcoholic fatty liver in rats

目的探讨柞蚕雄蛾液对酒精性脂肪肝的保护作用。方法将40只昆明种小鼠随机分为4组,柞蚕雄蛾大、小剂量组小鼠每日给予柞蚕雄蛾液(原液稀释度分别为1∶2、1∶4)和乙醇灌胃,20 d后所有小鼠在禁食12 h后取血及肝组织,检测血清TG、TC、ALT、AST浓度,常规病理学观察及脂肪肝改变评分;TBA法检测肝细胞线粒体MDA含量和SOD活性。结果模型组肝组织明显脂肪变性,ALT、AST浓度较正常组显著升高(P<0.05),MDA含量升高,SOD活性下降(P<0.05)。柞蚕雄蛾组与模型组比较肝组织脂肪变性明显减轻(P<0.05),小剂量组效果则更好(P<0.01);肝细胞线粒体MDA浓度降低(P<0.05),SOD活性升高(P<0.05);小剂量组ALT、AST浓度降低(P<0.05),大剂量组ALT、AST与模型组比较无显著性差异(P>0.05)。结论柞蚕雄蛾液能抵抗因饮用酒精而导致的肝细胞线粒体脂质过氧化,对乙醇诱导的肝脂肪变有一定保护作用。

Objective It is to observe the China oak silkworm liquid＇s function of obviating alcoholic fatty degeneration. Methods 40 Kunming rats were divided into 4 groups randomly. The China oak male silkworm liquid of high and low dosage was given to the rats in the treatment groups everyday （the dilution was 1：2 and 1：4）. 20 days later, all the rats were put to death 12 hour after fasting diet to get their vein blood and liver tissue. The tissue under microscope was observed and scored. TG, TC, ALT, AST in blood serum were determined, and the content of MDA, activity of SOD in mitochondria of liver cells were determined by TBA method. Results In model group, the fatty degeneration of liver tissue was obvious, and the levels of ALT, AST, MDA were obviously higher and the activity of SOD was lower than that in normal group（ P 〈 0.05）. Compared win model group, fatty degeneration in China oak silkworm liquid group was obviously improved （P 〈 0.05）, es- pecially in the low dosage group （P 〈 0.01）. The level of MDA lowed down and the activity of SOD rose up （P 〈 0.05）. Compared with model group, the contents of ALT and AST decreased in low dose group （P〈 0.05）, but in high dose group there was no significant difference （P 〉 0.05）. Conclusion The male China oak silkworm liquid can improve lipid peroxidation of mitochondria in liver cells, and has a certain extent protecting function on fatty degeneration induced by alcohol.