Nisin产生菌株的筛选及其鉴定

Isolation and identification of a Lactococcus lactis strain producing Nisin

以从传统乳制品中分离得到的7株乳酸菌为出发菌株,应用琼脂扩散法,以黄色微球菌为指示菌,筛选到了一株具有较大抑菌作用的乳酸菌。对S7产生的抑菌物质的物理化学性质研究表明,该抑菌物质的性质与Nisin一致;同时16SrDNA序列分析结果显示,S7是一株乳酸乳球菌,综合以上结果,可以认为这是一株Nisin产生菌。

A strain designated S7 which was isolated from the traditional dairy products was obtained using the agar-diffusion method employing Micrococcus flavus as an indicating strain. Further study on the inhibiting substance the S7 strain produced revealed that it had the same physicochemical characteristic with Nisin. Also, the 16SrDNA sequence indicated that S7 was a strain of Lactococcus lactic. SO it was concluded that a strain producing Nisin was obtained.