RP—HPLC法测定大叶紫珠中蔷薇酸的含量

RP -HPLC determination of euscaphic acid in Callicarpa macrophylla Vahl.

目的：建立测定民间常用药大叶紫珠中蔷薇酸含量的高效液相色谱法。方法：采用Kromasil—C18色谱柱（200mm×4．6mm，5μm），以乙腈-0．1％磷酸溶液（55：45）为流动相，1．0mL·min。为流速，207nm为检测波长。结果：大叶紫珠中蔷薇酸与其他成分能得到很好的分离，考察3个药用部位的大叶紫珠中蔷薇酸的含量，不同药用部位蔷薇酸含量差异较大，以大叶紫珠叶中蔷薇酸含量最高。测定线性范围为0．059～1．18mg·mL^-1，r=0．9999，平均回收率为98．4％（n=9）。结论：该法便捷、灵敏、准确，重复性好，可以控制该药材的质量。

Objective： To establish an RP - HPLC method for determination of euscaphic acid in Callicarpa macrophylla Vahl. ,which was commonly used in traditional medicine of Yunnan. Method：A Kromasil ClS column（200 mm ×4. 6 mm,5 μm） and a mobile phase consisted of acetonitrile-0. 1% phosphoric acid（55： 45）were used for analysis. The flow rate was 1.0 mL ·min ^- 1 and the UV detector wavelength was 207 nm. Results： Euscaphic acid was well separated from other compounds in Callicarpa macrophylla Vahl.. The contents of euscaphic acid in Callicarpa macrophylla Vahl. from different medicinal locus were significantly different. The calibration curve of euscaphic acid had a good linearity（ r =0. 9999）in the range of 0. 059 - 1.18 mg · mL^-1. The average recovery of euscaphic acid was 98. 4% （ n = 9 ）. Conclusion ： The method is simple, accurate, reproducible, which is suitable for the quality control of euscaphic acid in Callicarpa macrophylla Vahl..