RP-HPLC法同时测定复方明目胶囊中绿原酸和秦皮甲素的含量

RP-HPLC determination of chlorogenic acid and aesculin in compound Mingmu capsules

目的:建立复方明目胶囊中绿原酸和秦皮甲素含量的测定方法。方法:采用反相高效液相色谱法,ZORBAX～C_(18)(4.6mm×250 mm,5μm)色谱柱,乙腈-0.1%磷酸溶液(用三乙胺调节 pH=4.5)(8:92)为流动相,检测波长327 nm,流速0.8 mL·min^(-1),柱温25%。结果:绿原酸在1.0～6.0μg范围内与峰面积呈良好线性关系,r=0.9993,绿原酸平均加样回收率为100.2%,RSD 为0.9%(n=5);秦皮甲素在1.60～12.32μg范围内与峰面积呈良好线性关系,r=0.9996,秦皮甲素平均加样回收率为97.3%,RSD 为1.2%(n=5)。结论:本方法可靠性高,准确性和重复性好,适用于复方明目胶囊中绿原酸和秦皮甲素的含量测定。

Objective： To develop an HPLC method for determination of chlorogenic acid and aesculin in compound Mingmu capsules. Methods：The analytical column was ZORBAX -C18 （4. 6 mm× 250 mm ,5 μm）filled with a 5 μm stationary phase. The mobile phase consisted of acetonitrile - 0. 1% orthophosphoric acid （8： 92）, the flow -rate was 0. 8 mL · min^-1. The injection volume was 20μL. The detector wavelength was set at 327 nm. The column temperature was 25 ℃. Results：The precision and stability were fine. The calibration curve was linear over the range of 0. 1 -0.6 μg for chlorogenic acid（r =0. 9993） and 1.60 - 12. 32 μg for aesculin（r =0. 9996）. The average recovery （n = 5 ） of chlorogenic acid was 100. 2% and that of aesculin was 97. 3%. Conclusion：The proposed method may be used in the quantitative analysis of chlorogenic acid and aesculin. The method is convenient, economical and accurate with good reproducibility.