摘要
目的:建立 HPLC 法岩黄连注射液中岩黄连碱的含量。方法:Luna C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(1:1,1000 mL 含磷酸二氢钾3.4 g,十二烷基硫酸钠1.7g),流速1.0 mL·min^(-1),检测波长347 nm,柱温为室温。结果:岩黄连碱在5.48～49.34μg·mL^(-1)浓度范围内呈良好的线性关系(r=1.0000);方法平均加样回收率为99.96%,RSD=0.41%(n=6)。结论:本法可作为岩黄连注射液的质量控制方法。
Objective：To establish a method for the determination of dehydrocavidine in Yanhuanglian injection. Method： Chromatographic condition included a Luna C18 （250 mm ×4. 6 mm,5 μm）column and the mobile phase consisting of acetonitrile - water （ 1 ： 1, contained 3.4 g of potassium dihydrogen phosphate and 1.7 g of sodium laurylsulfate in 1000 mL）. The flow rate was 1.0 mL · min^-1. The column temperature was at room temperature and the detection wavelength was 347 nm. Results：The calibration curve was linear over the range of 5.48 -49. 34 μg · mL ^- 1 for dehydrocavidine （r = 1. 0000）. The average recovery was 99. 96%, RSD was 0. 41% （ n = 6 ）. Conclusion：The developed method can be used for quality control of Yanhuanglian iniection.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第6期904-906,共3页
Chinese Journal of Pharmaceutical Analysis
基金
广西科技厅资助课题(0448046)
