山莨菪碱、旋覆花素、苦碟子对过度训练大鼠肾细胞凋亡的影响

Effects of anisodamine, inula britannica and ixeris sonchifolia hance on the overtraining-induced renal cell apoptosis in rats

目的 评价山莨菪碱、旋覆花素、苦碟子对过度训练大鼠肾细胞凋亡的影响。方法 雄性Wistar大鼠72只，体重200-220g，随机分为5组：正常对照组（C组，n=8）、力竭对照组（E组，n= 16）、山莨菪碱组（A组，n=16）、旋覆花素组（IB组，n=16）、苦碟子组（IS组，n=16）。采用游泳至力竭建立过度训练动物模型。A组于力竭前即刻腹腔注射山莨菪碱10 mg／kg，IB组于力竭前24 h及力竭前即刻胃肠灌入旋覆花素25 ml／kg，IS组于力竭前即刻腹腔注射苦碟子20 ml／kg，力竭后6、24 h处死大鼠取肾脏标本，采用流式细胞术及TUNEL法检测肾细胞凋亡，并计算肾细胞凋亡率。结果 与C组比较，E组、A组、IB组和IS组力竭后6、24 h肾细胞凋亡率升高（P〈0.05或0．01）;与E组比较，A组、IB组和IS组力竭后6、24 h肾细胞凋亡率降低（P〈0．05）。结论 山莨菪碱、旋覆花素及苦碟子预先给药可减轻过度训练诱发大鼠肾细胞凋亡。

Objective To evaluate the effects of anisodamine, inula britannica and ixeris sonchifolia hance on renal cell apoptosis induced by overtraining in rats. Methods Seventy-two Wistar rats weighing 200-220 g were used in this study. Overtraining was induced by exhausting swimming. Exhaustion was defined as ： （ 1 ） the animal sank to the bottom 〉 10 s during swimming; （2） uncoordinated swimming and （3） the animal lost escape / righting reflex when placed on the table. The animals were randomly divided into 5 groups： group C control （ n = 8）, group E exhaustion （ n = 16） ,group A anisodamine （ n = 16）, group IB inula britannica （ n = 16） and group IS ixeris sonchifolia hance （ n = 16）. In group A and IS anisodamine 10 mg/kg and ixeris sonchifolia hance 20 ml/kg were given intraperitoneally immediately before exhausting swimming respectively. In group IB inula britannica 25 ml/kg was given orally 24 h and immediately before swimming. The animals were killed at 6 h and 24 h after exhaustion separately. The kidneies were obtained for determination of renal cell apoptosis by TUNEL and flow cytometry. Results The percentage of renal apoptotic cells was significantly increased in group E, A, IB and IS as compared with group C and was significantly lower in group A, IB and IS than in group E at 6 h and 24 h after exhaustion. Conclusion Pretreatment with anisodamine, inula britannica and ixeris sonchifolia hance can attenuate renal cell apoptosis in exhausted rats.