热休克蛋白HSP60与幽门螺杆菌在各型肠化胃黏膜组织表达的同步研究

Expression of HSP60 and H.pylori in gastric mucosa with intestinalmetaplasia

目的:探讨热休克蛋白60(heat shock proteins60,HSP60)在各型肠化胃黏膜组织中的表达及与H.pylori感染之间的相互关系。方法:采用SP免疫组织化学技术进行胃黏膜HSP60的检测,用组织吉姆萨染色、H.pylori IgG抗体检测及Hp-DNAPCR检测三种方法进行H.pylori感染情况的判定。结果:HSP60在肠化胃黏膜组织中主要为中等强度阳性表达,Ⅰ、Ⅱ和Ⅲ型肠化胃黏膜组织的阳性率分别为35.2%、51.3%和75.6%,过表达率分别为1.9%、4.0%和19.5%。浅表性胃炎组织中的表达多为弱阳性或中等强度阳性,阳性表达率为30.0%。胃癌组织的HSP60表达以强阳性和中等强度阳性为主,阳性表达率为73.5%,过表达率为34.7%。各型肠化胃黏膜组织HSP60总的阳性表达率显著高于浅表性胃炎组,Ⅰ型肠化胃黏膜组织的阳性表达率与浅表性胃炎无差异,Ⅱ和Ⅲ型肠化胃黏膜组织HSP60的表达阳性率明显高于浅表性胃炎,Ⅰ和Ⅱ型肠化胃黏膜组织的HSP60表达率明显低于胃癌组,Ⅲ型肠化胃黏膜组织的表达阳性率与胃癌组无差异。结论:提示HSP60的表达及表达强度发生在肠化胃黏膜病变各型进展和癌变过程中;肠化胃黏膜组织H.pylori感染和HSP60表达有明显的相关性。

OBJECTIVE： To study the expression of heat shock protein60 （HSP60） in gastric mucosa with different types of intestinal metaplasia （IM） and discuss the relationship between the expression of HSP60 and H. pylori infection. METHODS： HSP60 in gastric mucosa was detected by SP immunohistochemistry, and H. pylori infection was detected by Giemsa＇ s staining, serum H. pylori IgG and the Hp-DNA PCR detection. RESULTS： HSP60 expressed medium intensity positive in type Ⅰ, type Ⅱ and type Ⅲ IM gastric mucosa, the positive rates were 35.2%, 51.3% and 75.6%, respectively, and the overexpression rates were 1.9%, 4.0% and 19.5%, respectively. The HSP60 expression in superficial gastritis was weak or medium intensity positive, and the positive rate was 30.0%. The HSP60 expression in gastric cancer was mainly strong positive and medium intensity positive, with the positive rate 73.5%, overexpression rate 34. 7%. The total positive expression rate of HSP60 in IM gastric mucosa is much higher than that in superficial gastritis. The positive expression rate of HSP60 in type Ⅰ IM gastric mucosa and superficial gastritis has no difference. The positive expression rate of HSP60 in type Ⅱ , type Ⅲ IM gastric rnucosa is increased compared with that of superficial gastritis. HSP60 expression rate in gastric cancer is elevated significantly compared with that of type Ⅰ and type Ⅱ IM , but no difference is found compared with that of type Ⅲ IM mucosa. CONCLUSIONS： That HSP60 expression and expression intensity occurs in the progressing of IM gastric mucosa and gastric cancer. The H. pylori infection of IM gastric mucosa has relationship with the expression of HSP60.