星形细胞瘤抗原处理相关转运蛋白异常表达的临床意义

Abnormal expression of TAP gene and its clinical significant in astrocytoma

目的:研究星形细胞瘤中抗原处理相关处理蛋白(TAP)分子表达及其临床意义,探讨星形细胞瘤的治疗对策。方法:采用免疫组织化学技术检测50例星形细胞瘤患者肿瘤组织和15例正常脑组织中TAP的表达。结果:50例星形细胞瘤患者中27例TAP蛋白表达阳性,23例TAP蛋白表达阴性,TAP蛋白阳性细胞百分率为29.1%±27.24%;正常组15例,全部表现为TAP蛋白表达阳性,TAP蛋白阳性细胞百分率为58.4%±21.69%,两组均数比较差异有统计学意义,t=3.8110,P<0.05。低级别星形细胞瘤(Ⅰ级和Ⅱ级)25例,TAP蛋白平均阳性细胞百分率为37.4%±29.13%;高级别星形细胞瘤(Ⅲ级和Ⅳ级)25例,TAP蛋白平均阳性细胞百分率为22.8%±20.08%;两组均数比较差异有统计学意义,t=2.0631,P<0.05。结论:星形细胞瘤患者存在TAP分子低表达,这可能促使星形细胞瘤患者肿瘤细胞逃避免疫监视,TAP蛋白缺失率可作为预测脑星形细胞瘤的发生及其恶性程度的指标之一。

OBJECTIVE： To study the expression of TAP protein by SP immunohistochemical analysis and to discuss the correlation between TAP protein and pathological grade, and investigate the value of TAP in evaluating malignancy. METHODS： There were two groups in the experiment, and the cases included 15 cases of normal group and 50 cases of tumor group. All the samples were hydrated through graded alcohols, then the antigens were repaired by microwave, and the expression of TAP protein was determined by SP immunohistochemical analysis. RESULTS： Of the 50 cases of astrocytoma, 27 cases were positively expressed with TAP protein and the percentage of TAP protein was 29. 1%±27.24%. Among the 15 normal cases, all were positively expressed with TAP protein and the percentage of TAP protein was 58.4%±21.69%. The percentage of TAP protein was 37.4%±29. 13% in the 25 cases of low grade astrocytoma, and it was 22. 8%±20.08% in the 25 cases with high grade astrocytoma. There were significant differences in the two groups, P〈 0. 05. CONCLUSION： The underexpress of TAP may be an important cause for the development of astrocytoma.