幽门螺杆菌感染与胃癌危险性关系的研究

STUDY ON THE RELATIONSHIP BETWEEN HELICOBACTER PYLORI INFECTION AND RISK OF GASTRIC CANCER.

研究幽门螺杆菌（Ｈｐ）感染是否增加患胃癌的危险性。以流式细胞术、增殖细胞核抗原免疫组化和核仁组成区嗜银蛋白银染技术研究胃粘膜细胞增殖活性，以硫代巴比妥酸比色法测定过氧化脂质含量，以荧光法测定ＤＮＡ损伤程度，观察Ｈｐ清除前后胃粘膜细胞动力学、脂质过氧化水平和ＤＮＡ损伤等胃癌易感标志的变化。Ｈｐ阳性患者胃粘膜细胞增殖指数、增殖细胞核抗原标记指数和核仁组成区嗜银蛋白数均显著增加，ＤＮＡ－ＥＢ荧光强度显著降低；胃粘膜过氧化脂质含量显著增加，且与ＤＮＡ－ＥＢ荧光强度呈显著负相关。经治疗Ｈｐ被清除后，上述指标均明显改善，而Ｈｐ仍为阳性者，上述指标则无显著性变化。Ｈｐ感染的胃粘膜具有较高的癌变易感性，Ｈｐ感染是胃癌发生的危险因素之一。

investigate the relationship between Helicobacter pylori(HP)infection and risk of gastric cancer.Cell kinetics,Lipid peroxidation and DNA damage of gastric mucosa were studied before and after Hp clearance.Cell proliferative kinetics were evaluated by flow cytometry,proliferative cell nucleolar antigen(PCNA) immunohistochemical method and nucleolar organizer regions(AgNORs)silver stain method.DNA damage was determined by fluorescent method using EB as the fluorescent prob.Proliferative index,PCNA labeling index and AgNORs were significantly incereased in Hp infected gastric mucosa and significantly decreased after Hp clearance with triple therapy.While the fluorescence intensity of DNA EB complex in Hp infected gastric mucosa was significantly decreased and was significantyly increased after Hp clearance.The changes in lipid peroxide were similar to that in DNA damage.Hp infected gastric mucosa was more susceptible to gastric cancer,Hp infection may be one of the risk factors of gastric carcinogenesis.