食管鳞癌组织中线粒体DNA拷贝数量的变化及意义

Change in the Mitochondrial DNA Copy Number and its Significance in Esophageal Squamous Cell Carcinoma

目的:比较线粒体DNA(mitochondrial DNA,mtDNA)拷贝数量在食管鳞癌、癌旁粘膜和正常食管粘膜组织间的差异,探讨mtDNA与食管鳞癌发生、发展的关系。方法:应用荧光定量PCR(real-time PCR)技术,定量检测42例食管鳞癌、癌旁粘膜和正常食管粘膜组织中mtDNA拷贝数;并采用凝胶电泳对mtDNA进行定性检测。结果:食管鳞癌组织中mtDNA检测率为100%(42/42),平均拷贝数为27.1894×106μgDNA;正常食管粘膜组织mtDNA检测率100%(42/42),平均拷贝数为5.9347×106μgDNA;癌旁粘膜组织mtDNA检测率100%(42/42),平均拷贝数为9.4102×106μgDNA,三者之间有显著差异(F=27.83,P<0.05)。PCR产物经凝胶电泳后,在403bp处显示阳性条带,且食管鳞癌组织所在的泳道较亮,与real-timePCR检测结果一致。结论:mtDNA拷贝数量的增加与食管鳞癌的发生、发展有关。

Objective： To compare the difference in mitochondrial DNA （mtDNA） copy number in esophageal squamous cell carcinoma （ESCC）, paraneoplastic tissue and normal mucous membrane of the esophagus and to study the relationship between the mtDNA copy number and the occurrence and de- velopment of esophageal squamous cell carcinoma. Methods： The mtDNA copy number of 42 speci- mens of ESCC, paraneoplastic mucous tissue and normal mucous membrane of the esophagus was determined using real-time fluorescent quantitative PCR. Qualitative tests of the mtDNA were conducted using agarose gel electrophoresis. Results： The rate of successful mtDNA amplification was 100% （42/ 42） for ESGG, paraneoplastic tissue and normal mucous membrane of the esophagus, with the average mtDNA copy number detected at 27.189 4×10^6μg DNA, 9.410 2×10^6μg DNA and 5.934 7×10^6μg DNA, respectively. There were significant differences （F=27.83,P〈0.05） in mtDNA copy number among the three. A strong band was detected at 403bp after gel electrophoresis of the PCR products, and the track where the amplicons from the ESCC tissues were loaded was rather bright, which was in accordance with the results of real-time PCR. Conclusion： Incremental increases in the mtDNA copy number is related to occurrence and development of ESCC.