尖锐湿疣组织学诊断与病原学检测的探讨

Histopathologic diagnosis of condyloma acuminata and detection of the etiology viruses

根据人类乳头瘤病毒感染外阴皮肤粘膜后的组织学改变特征诊断尖锐湿疣，抽取３１例行病毒核壳抗原（ＨＰＶ－Ａｇ）免疫组化染色和ＰＣＲ检测ＨＰＶＤＮＡ。结果：ＨＰＶ－Ａｇ的检出率为８３．８７％（２６／３１），ＨＰＶＤＮＡ的检出率为１００％（３１／３１）。提示，结合病毒的致病机理，掌握尖锐湿疣的组织病理改变要点，能够对该病作出准确的诊断。

Capsid antigen of human papilloma virus(HPV Ag) were detected by histoimmunologic technique and HPV DNA by polymerase chain reaction (PCR) in 31 cases of condyloma acuminata. 83.87% (26/31) cases showed positive results of HPV Ag, 100% (31/31) cases were positive for HPV DNA detection. Correct diagnosis could be made based on the histopathologic characteritics of human papilloma virus infection.