利用CDR_3谱型分析法检测T细胞群的克隆性扩增

ANALYSIS OF CLONAL PREDOMINANCE OF T CELL RECEPTORS BY CDR 3 SIZE SPECTRATYPING

介绍一种快速、简便检测Ｔ细胞群克隆性扩增的方法。Ｔ细胞抗原受体（ＴＣＲ）α和β链与抗原／ＭＨＣ复合物相互反应是由ＣＤＲ１、ＣＤＲ２和ＣＤＲ３环完成的；其中ＣＤＲ３环是由Ｖ－（Ｄ）－Ｊ片断所组成直接与抗原相结合，因此长度及序列在不同的Ｔ细胞克隆之间均有变化，而单一Ｔ细胞克隆则长度、序列是一致的。应用ＰＣＲ技术分析ＴＣＲＶβ家族中ＣＤＲ３的长度谱型可检测样品中Ｔ细胞克隆特异性。为研究感染性疾病及自身免疫病末梢血及病灶组织Ｔ细胞克隆性扩增提供快速、经济、可行的方法。

Structural models for the TCR α/β predict that CDR 1,CDR 2 and CDR 3 loops of both the α and β chains contribute to specific interactions with the Ag/MHC complex. The CDR 3 loops are constructed by joining events invo ving the V (D) J segments,and thus may vary in both sequence and length. We developed a polymerase chain reaction assay to assess the lenngth variation of the CDR 3 loop in TCR derived from Vβ segments families in peripheral blood T cells,T cell clone and brain samples. The result showed that spectrotype analysis is applicable to the studies of specific repertoire skewing such as may be associated with inflammatory and autoimune disorders.