川芎嗪对大鼠脑缺血再灌注损伤的保护作用

Protective Effect of Ligustrazine on Cerbral Ischemia-reperfusion Injury of Rats

目的:观察川芎嗪对大鼠脑缺血再灌注损伤的保护作用。方法:采用线栓法复制大鼠大脑中动脉脑缺血再灌注损伤模型,检测血液流变学指标,并测定再灌注后缺血侧脑组织中含水量、丙二醛(MDA)含量、总抗氧化能力(T-AOC)和超氧化物歧化酶(SOD)活性。结果:川芎嗪60mg/kg、30mg/kg、15mg/kg剂量均不同程度降低高、中、低切变率全血黏度、红细胞压积和血沉,能拮抗缺血脑组织脑含水量和MDA含量的增加,提高脑组织T-AOC、SOD含量。结论:川芎嗪对大鼠大脑中动脉缺血再灌注损伤具有明显保护作用。

Objective：To study the protective effect of Ligustrazine （Lig） against cerbral ischema -reperfusion injury in rats. Method： SD rats were treated with Lig for 6 days and were prepared to focal ischemia with middle cerebral artery occlusion （MCAO）. After 2 hours ,perfusion was recovered by pulling out the suture. 12 hours later,hemorrheology indexes were measured and the homogenate of brain tissue were obtained after reperfusion. The contents of water and malondialdehyde （MDA） levels, total antioxidant capacity（ T -AOC） and the activities of superoxlde disutase （SOD） and （T- AOC） were determined by spectroscopy. Results： Hemorheological research it showed that Lig reduced whole blood viscosity, hematocrit （HCT）, erythrocyte sedimentation rate （ESR）. Lig decreased the contents of water and MDA, elevated the activities of SOD and T- AOC. Conclusion： It is suggested that Lig can improve hemorheological state and protect against cerebral ischemia - reperfusion injury by enhancing the activity of anti - oxidative enzymes, inhibiting lipid peroxidation subsequently and attenuating free radicals - mediated brain damage.