雷丸蛋白酶的发酵、提取及对猪囊尾蚴体外活性作用的初步研究

Fermentation and extraction of Leiwan(Omphalia lapidescens Schroet) proteinase and its activation against cysticercus cellulosae in vitro

目的：探索雷丸菌发酵产物中是否含有和天然雷丸中相同分子量的而且具有体外杀灭猪囊尾蚴活性的雷丸蛋白酶。方法：对雷丸菌发酵所产生的蛋白酶进行提取、检测和体外杀灭猪囊尾蚴的实验，并和天然雷丸蛋白酶进行比较。结果：从雷丸菌发酵产物中分离出来的雷丸蛋白酶经测定，分子量为１６８００，等电点为４．４，末端氨基酸为缬氨酸，具有水解蛋白的活力及体外杀灭猪囊尾蚴的活性。结论：采用雷丸菌发酵方法所获得的雷丸蛋白酶与天然雷丸蛋白酶之间没有明显差别。

OBJECTIVE:To investigate whether the fermentation products of Omphalia lapidescens Schroet strain contain Leiwan proteinase with molecular weight and parasiticide effect on cysticercus cellulosae in vitro both similar to these of natural Leiwan.METHODS:We examined the fermentation products of Omphalia lapidescens Schroet strain,and tested the parasiticide effect on cysticercus cellulosae in vitro,in comparison with the natural Leiwan proteinase.RESULTS:In the Leiwan proteinase extracted from the fermentation products of Omphalia lapidescens Schroet strain,the molecular weight was 16 800,PI 4.4,and amino terminal valine.It had proteolysis activity and parasiticide effect on cysticercus cellulosae in vitro.CONCLUSION:There were no remarkable differences between the Leiwan proteinase obtained by the method of Omphalia lapidescens Schroet strain fermenting,and the natural Leiwan proteinase.