固相萃取-薄层扫描法测定血清中青藤碱浓度

Determination of sinomenine in serum by thin layer chromatography using solid phasc extraction

目的：研究血清中青藤碱的液固净化技术和在家兔体内的动力学特征。方法：自制硅胶萃取柱及萃取仪，将血清中青藤碱净化后，点样于硅胶Ｇ硅胶ＧＦ２５４（２∶１）薄层板上，以氯仿甲醇（１９∶２）为展开剂，在岛津ＣＳ９３０上，用双波长（λＳ２７５ｎｍ，λＲ３２０ｎｍ）锯齿法扫描测定。结果：血清中青藤碱的固相提取回收率为９６．９５％±ｓ７．５９％，用薄层扫描法测绘了２只家兔静注青藤碱后的药物浓度时间曲线。结论：自制萃取柱净化血清样品简便、有效；青藤碱的薄层扫描测定法灵敏、准确。

OBJECTIVE:To study the purification of sinomenine in serum by the liquid solid extraction and the pharmacokinetics of sinomenine in rabbits.METHODS:To sinomenine in serum was extracted by silica column which made in our laboratory and redissolved in CHCl 3.The sample was applied on silica GF 254 plate and developed with CHCl 3 CH 3OH(19∶2).The spot was detected by CS 930 at λ S=275 nm,λ R=320 nm.RESULTS:The average recovery was 96.95%±s 7.59 % for SPE(solid phase extraction) procedure in serum.The drug time curve of sinomenine in rabbits was determined.CONCLUSION:The SPE columns which made in our laboratory are simple and efficient for the purification of serum samples.The TLC is sensitive and precise for the determination of sinomenine.