肺癌患者肺泡巨噬细胞精氨酸酶活性研究

STUDY OF ARGINASE ACTIVITY IN ALVEOLAR MACROPHAGES FROM PATIENTS WITH LUNG CANCER

检测肺泡巨噬细胞（ＡＭ）培养液中精氨酸酶活性。结果表明，肺癌患者荷瘤侧肺ＡＭ较非荷瘤侧肺和非癌性肺病患者ＡＭ产生精氨酸酶活性低；ＡＭ经卡介菌（ＢＣＧ），干扰素－α（ＩＦＮ－α）刺激后产生精氨酸酶活性明显增强。提示肺癌患者肿瘤局部ＡＭ抗肿瘤活性存在缺陷；ＢＣＧ，ＩＦＮ－α诱导ＡＭ产生精氨酸酶可能为其抗肿瘤、抗感染的机制之一。

Alveolar macrophages (AM) were obtained from 20 patients with primary lung cancer and 20 cases with nonmaligment pulmonary disease by BAL and were incubated in vitro in medium with and without BCG and/or IFN α. Then the cell free supernatants were harvested. The activity of arginase was assayed. It was found that: arginase was produced spontaneously by AM; The spontaneous production of arginase is lower in AMs from the tumor bearing segments than either nontumor bearing segments or AMs from the patients with nonmalignant pulmonary diseases ( P <0,.05). The production of arginase was icreased on AMs from the tumor bearing segments stimulated with BCG and/or IFN α ( P <0.05 or 0.01). The results suggested that (1) There are some function at defects of tumor. (2) The mechanism of anti tumor and anti infection activity of BCG and IFN α may possibly increase the production of arginase of AMs.