碱性成纤维细胞生长因子对牛眼晶体上皮细胞的促增殖作用

Promotive effects of basic fibroblast growth factor on proliferaton of bovine lens spithelial cells

目的观察碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）对牛眼晶体上皮细胞的作用。方法首先进行牛眼晶体上皮细胞原代与传代培养；在第２代培养细胞中添加ｂＦＧＦ，浓度１０－２～１０２ｎｇ／ｍｌ，借助甲基噻唑基四唑（ｍｅｔｈｙｌｔｈｉａｚｏｌｙｌｔｅｔｒａｚｏｌｉｕｍ，ＭＴＴ）测定方法观察ｂＦＧＦ对晶体上皮细胞增殖的影响。结果牛眼晶体上皮细胞体外具有易于贴附、细胞融合较快以及生长迅速等特点。添加ｂＦＧＦ后，可促进晶体上皮细胞的增殖，尤其是１０２ｎｇ／ｍｌ显示出明显的促增殖作用。结论ｂＦＧＦ在促进白内障术后晶体上皮细胞的增殖及后囊混浊上起了重要作用。

Objective In order to observe the effect of basic fibroblast growth factor (bFCF) on bovine lens epithelial cell growth.Methods Primary culture and subculture of bovine lens epithelial cells were established in vitro first and the bFCF concentrated form 10 -2 ng/ml to 10 2ng/ml was added to the epithelial cells of second passage respectivedly.The proliferative charactristics of the cells were measured with methyl thiazolyl tetrazolium (MTT) assay method. Results It is indicated that in vitro bovine epithelial cells are characterized by easy adherence, rapid confluence,high growth,etc. After the treatment with bFGF,lens epithelial cells showed marked proliferation depending on the concentration of bFGF from 1 ng/ml to 10 2ng/ml,especially in 10 2 ng/ml of bFGF. Conclusion It is suggested that bFGF play an important role in lens epithelial cell proliferation and posterior capsular opacities after cataract surgery.