人工晶体缝线固定术睫状沟组织的免疫组织化学研究

An immunohistochemical study on ciliary sulcus tissue in cases of intraocular lens implantation with ciliary sulcus suture fixation

目的观察后房型人工晶体睫状沟缝线固定术是否造成兔眼睫状沟组织细胞异常增殖。方法用９６只新西兰白兔建立动物模型，行后房型人工晶体睫状沟缝线固定和单纯固定术。分别于术后１／２、１、２、３个月摘除眼球，用免疫组织化学方法观察两种术式及正常眼（对照组）睫状沟部位组织细胞的增殖细胞核抗原反应。结果两种术式均未导致睫状沟组织细胞的异常增殖反应。结论睫状沟缝线固定术不失为处理后房型人工晶体植入术中后囊破裂及二期植入的可行术式。

Objective To observe abnormal cell multiplication in rabbits′ ciliary sulcus with posterior chamber intraoculr lens (PC IOL) implantation and ciliary sulcus fixation technique.Methods 96 New Zealand rabbits were implanted with PC IOL,and they were divided into two groups:one with ciliary sulcus suture fixation and the other without the suture fixation.The eyeballs wrer extracted at the following postoperative times:1/2,1,2,3 months.The reactions of the proliferating cell nuclear antigen(PCNA) in ciliary sulcus of the rabbits were studied with immunohistochemistry technique in the above two groups and the normal control group. Results There was no abnormal proliferating cell reaction in ciliary sulcus of the rabbits with the two methods of PC IOL implantation techniques.Conclusions IOL ciliary sulcus suture fixation is a convenient method for PC IOL implantation under the circumstance when rupture of the posterior capsule occurs intraoperatively or in the second stage implantation.