北京儿童医院下呼吸道感染住院患儿血清腺病毒抗体检测分析

Detection of adenovirus-IgM antibody in children hospitalized with lower respiratory tract infection in Beijing Children's Hospital

目的了解北京儿童医院急性下呼吸道感染住院患儿的腺病毒(ADV)感染情况。方法研究对象为北京儿童医院2004年10月至2005年12月临床诊断为肺炎或支气管炎的住院患儿。每例患儿的血清标本采用间接酶联免疫吸附试验检测ADV-IgM抗体。如果任意一种其他病毒抗体阳性(包括巨细胞病毒、EB病毒、单纯疱疹病毒、肠道病毒等),则不做ADV检测。结果738例其他病原检测阴性的下呼吸道住院患儿中,ADV-IgM阳性87例,阳性率11.8%。ADV下呼吸道感染占同期病例的8.3%(87/1046)。其中<1岁的患儿32例(6.2%);1～6岁42例(29.8%);>6岁13例(16.9%)。ADV感染全年散发,冬春季节相对较多。结论ADV是5岁以下儿童急性呼吸道疾病的主要病原之一;6月龄至3岁的儿童为易感人群。

Objective To explore the characteristics of adenovirus （ADV） infection in children hospitalized with lower respiratory tract infection （LRTI） in Beijing Children＇ s Hospital. Methods One thousand and forty-six children hospitalized with pneumonia or bronchitis in Beijing Children＇ s Hospital from October 2004 to December 2005, were enrolled in our study. Serum samples were collected from each patient and detected for ADV-IgM by indirect ELISA, excluding those serum samples which were positive for any of the antibodies as CMV,EBV,HSV,Enterovirus. Results Eighty-seven （ 11.8% ,87/738） were positive for ADV-IgM in 738 cases which were negative on other pathogen. The percentage of LRTI with ADV infection was 8.3 % （87/1046） during the period. Thirty-two cases were younger than 1 year of age while 42 cases were between 1-6 and 13 cases were older than 6 years old. The ratio of male to female was 1.4 ： 1. ADV infection occurred throughout the year and relatively frequent in winter and spring. Conclusion ADV was one of the most important viral pathogens on lower respiratory tract infections in children under 5 year olds. Children aged from 6 months to 3-years were susceptible to ADV infection.