硝酸盐还原试验快速检测分离株和痰标本结核分枝杆菌的耐药性

Rapid testing on drug susceptibility of Mycobacterium tuberculosis with nitrate reducrase assay

目的建立一种快速结核分枝杆菌耐药性试验方法。方法以绝对浓度法为"金标准",对已知耐药浓度的耐药结核分枝杆菌菌株及敏感株采用硝酸盐还原试验检测其对利福平和异烟肼的耐药性。同时用抗酸染色阳性的痰标本经常规处理后进行硝酸盐还原试验。结果结核分枝杆菌分离株两种方法可比性好.与绝对浓度法比较,硝酸盐还原试验敏感性为96.5%以上,特异性100%;该方法比传统绝对浓度法平均提前3周有结果;硝酸盐还原法的平行试验表明,试验重复性好,符合率为100%。痰标本硝酸盐还原试验在第10-20天能有结果,与痰标本罗氏培养后的分离株的绝对浓度法比较,硝酸盐还原试验敏感性为66.7%以上,特异性100%。结论硝酸盐还原试验可作为快速结核分枝杆菌耐药性试验方法。

Objective To establish a rapid method for testing drug sussceptibility on Mycobacterium tuberculosis. Methods Taking absolute Concentration method for drug susceptibility testing of M. tuberculosis as the ＂gold standard＂, we examined the drug-resistant of M. tuberculosis strain with nitrate reducrase assay （NRA） and the drug-resistant of M. tuberculosis germ in sputum with NRA. Results NRA and absolute concentration method was basically comparable with NRA susceptibility as 96.5% and the specificity was 100%, When comparing with traditional absolute concentration method, NRA could shorten the time about 3 weeks. Using NRA to test the drug-resistant of M. tuberculosis germ in sputum, its susceptibility was more than 66.7% and specificity was 100%, within 10-20 days. Conclusion NRA could be used as a rapid drug susceptibility testing on M. tuberculosis.