摘要
用卡拉胶将肌红蛋白(Mb)固定在玻碳电极(GC)表面,制备了MB-卡拉胶膜修饰电极.包埋在卡拉胶中的Mb可以与电极发生直接电子传递.Mb-卡拉胶膜修饰电极在水/有机混合溶液中,表现出准可逆的直接电化学过程.式电势(E0’=-0.407V)表明该电化学响应是MbFe(III)/Fe(II)电对的氧化还原.在一定范围内,溶液pH增加,Mb氧化还原式电势负移,且呈线性关系,说明Mb的电子传递过程伴随有质子的转移.扫描电镜显微研究表明,卡拉胶水凝胶与肌红蛋白之间有较强的作用,紫外光谱表明,血红素周围的微环境没有改变.
Myoglobin (Mb) was immobilized on glassy carbon electrode (GCE) surface by carrageenan. Mb entrapped in the carrageenan film undergoes fast direct transfer-electron reactions in aqueous-organic solvents mixtures. The formal potential (E^0'=-0.407V) indicate the redox of MbFe(Ⅲ)/(Ⅱ) couple. The E^0'is linearly dependent on solution pH (redox Bohr effect), indicating the electron transfer of MbFe(Ⅲ)/(Ⅱ) redox couple companied with the transfer of proton. The morphologies of scanning electron microscopy indicate the interaction between carrageenan and Mb. The UV-Visible absorption spectra show the conformation of poly peptide around the heme region unchanged.
出处
《襄樊学院学报》
2007年第5期41-44,共4页
Journal of Xiangfan University
基金
湖北省自然科学基金项目(2005ABA007)
湖北省教育厅科技基金项目(G200525001)
关键词
肌红蛋白
卡拉胶
直接电化学
Myoglobin
Carrageenan
Direct electrochemistry