摘要
目的观察糖尿病大鼠心肌病变与基质细胞衍生因子-1α(SDF-1α)及其受体(CXCR-4)的关系。方法以链脲佐菌素(STZ)诱导建立糖尿病大鼠模型。随机分为1个月病程组(M1组)、3个月病程组(M3组)、5个月病程组(M5组)。心肌切片后行SDF-1α,CXCR-4及血管内皮生长因子(VEGF)免疫组化,抗vWF抗体染色心肌微血管计数,半定量反转录聚合酶链反应(RT-PCR)检测SDF-1α,CXCR-4及VEGFmRNA在大鼠心肌中的表达水平。结果随着病程的延长,心肌组织中SDF-1α,CXCR-4表达水平逐渐增高,与正常对照组相比,造模后1个月就有增高,5个月时增高最明显,而心肌组织中VEGF表达无明显改变;心肌微血管计数显示造模后5个月心肌内微血管数量明显减少。结论糖尿病心肌病变时SDF-1α/CXCR-4在心肌中的表达水平随糖尿病病程延长逐步增高,而VEGF表达无明显改变,糖尿病心肌病变时微血管减少可能与VEGF表达不足有关。
Objective To study the relationship between features of myocardium in diabetic rats and SDF- 1α and its receptor （ chemotaxis cytokine receptor - 4, CXCR - 4）. Methods Diabetes models were induced in 60 rats with a single intraperitional injection of streptozotocin （STZ）. Experimential rats were randomly divided into M1 （diabetic for 1 month）, M3 （diabetic for 3 months）, M5 （diabetic for 5 months） groups, and other 30 rats were assigned as normal control group （NC）. After myocardium slicing, SDF- 1α, CXCR -4 and VEGF immunohistochemistry were tested. Myocardium SDF - 1α , CXCR - 4 and VEGF mRNA were detected by semi - quantitative RT- PIER. The changes of myocardium microvessel density （MVD） were observed by anti - vWF staining. Results With the prolongation of the duration of disease, the expression of SDF- 1α, CXCR - 4 on myocardium increased gradually. But the expression of VEGF had no apparent change. The MVD decreased in M5 group. Conclusion The experession of SDF - 1α and CXCR - 4 on myocardium in diabetic rat with cardiomyopathy increased gradually with the prolongation of the duration of disease. But the expression of VEGF had no apparefit change.
出处
《中西医结合心脑血管病杂志》
2007年第7期601-603,共3页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
