一种简易大鼠蛛网膜下隙出血模型的建立

A SIMPLE MODEL OF SUBARACHNOID HEMORRHAGE IN RATS

目的建立一种操作简单、重复性好的蛛网膜下隙出血(SAH)动物模型。方法42只Wistar大鼠随机分为SAH组、假手术组和正常对照组。SAH组鼠尾取血(用自制套管针控制注血深度)注入枕大池造成SAH,假手术组注入等量的生理盐水,正常对照组不做处理。以LOEFFLE神经行为学评分方法和解剖学观察进行模型的评价。结果SAH组各时点神经行为学评分明显低于假手术组相应各时点(t=4.78～10.30,P<0.01)。正常对照组及假手术组大鼠蛛网膜下隙未见积血,SAH组大鼠枕大池见片状积血。结论枕大池注血法建立SAH大鼠模型简单、可靠,重复性好。

Objective To build a simple animal model of subarachnoid hemorrhage. Methods Forty-two Wistar rats were randomly divided into three groups： SAH group, sham-operation group and control group. The rats in SAH group were produced by blood taken from the taft of rats then injected into cisterna magna, and those in the sham-operation group were injected with saline of the same volume, the normal control group without arty injection. The assessment of the models were done by Lodflie＇s neurobehavior score and the anatomy was observed as well. Results The neurobehavior scores in sham-operation group were higher than those of SAH group （t=4.78-10.30,P〈0.01）. There was always blood in the basal cisterns with some spread over the hemisphere in rats of SAH group, it was not seen in control group and sham-operation group. Conclusion The establishment of SAH-rat models using autoblood is simple, reliable and repeatable.