Epo及其受体在宫颈癌及癌前病变组织的表达

EXPRESSIONS OF ERYTHROPOIETIN AND ERYTHROPOIETIN RECEPTOR IN CERVICAL CANCER AND PRECANCEROUS LESION

目的了解促红细胞生成素(Epo)及其受体(EpoR)在宫颈癌及其癌前病变组织的表达及其意义。方法用半定量RT-PCR法,检测30例宫颈癌组织Epo、EpoR mRNA含量,用免疫组化PV-6000方法检测50例宫颈癌及其癌前病变组织Epo、EpoR蛋白表达,并与正常宫颈组织比较。结果Epo、EpoR mRNA在宫颈癌及正常宫颈组织中均有表达,在宫颈癌组织中的表达水平较高,各组间比较差异有显著性(F=11.83、173.73,q=4.83、10.86,P<0.05)。Epo、EpoR蛋白表达均为阳性,各组间表达率比较,差异有显著性(χ2=5.78、15.88,P<0.05),Epo、EpoR蛋白表达率随宫颈病变程度的加重明显增加;EpoR蛋白表达与Epo蛋白表达呈显著正相关(r=0.57,P<0.01)。结论Epo/EpoR的表达可能与宫颈癌发生发展有关。

Objective To investigate the expressions of erythropoietin （Epo） and erythropoietin receptor （EpoR） in cervical cancer and precancerous lesion, Methods Epo and EpoR mRNA in 30 cases of cervical cancer were determined by semiquantitative RT-PCR, and the protein expressions of Epo and EpoR in 50 cases of cervical cancer and precancerous lesion were analyzed by immunohistochemistry PV-6000. Results mRNA expressions of Epo and EpoR were found in both cervical cancer and normal cervix,with the expressions in cervical cancer significantly higher. The differences in Epo and EpoR mRNA expressions among different cervical cancer groups were significant （F= 11. 83,173.73 ; q= 4, 83,10.86 ;P〈0.05）, Positive staining of Epo and EpoR protein was found in all groups, the differences among different groups were significant （x^2 =5.78,15.88; P〈0.05）. The Epo and EpoR increased along with the severity of the lesions. The EpoR expression was higher than Epo in the other groups. There was a positive correlation betweem Epo and EpoR （r=0.57,P〈0.01）. Conclusion Epo and EpoR may play an important role in the development of cervical cancer.