人发角蛋白人工神经的免疫学研究

IMMUNOLOGICAL STUDY OF HUMAN HAIR KERATIN ARTIFICIAL NERVE

目的探讨人发角蛋白(HHK)人工神经组织相容性,为周围神经(PN)缺损寻求理想的移植修复材料。方法54只健康成年SPF级雄性Wistar大白鼠随机分为A、B、C三组。A组为实验组,B、C组为对照组。将各组大白鼠右侧胫神经切除10 mm,A组用人发角蛋白人工神经桥接;B组将切除神经倒转180°原位缝合;C组用A组大白鼠所切除的胫神经桥接。术后2、8、16周各组随机取6只大白鼠,采血检测免疫球蛋白IgAI、gM、IgG,补体C3、C4,T淋巴细胞亚群及T淋巴细胞转化率。结果术后2、8、16周各组血液免疫学指标比较,A、B两组之间均无显著性差别(F=17.56～571.34,q=0.00～2.09,P>0.05),C组与A、B两组比较均有显著性差别(q=7.04～41.47,P<0.01)。结论人发角蛋白人工神经具有良好的组织相容性,是修复PN缺损较为理想的移植材料。

Objective To investigate the histocompatibility of human-hair keratin （HHK） artificial nerve and offer an ideally transplanted material for repairing the defect of peripheral nerve （PN）. Methods Fifty-four Wistar rats were randomly divided into three groups： group A, experiment group; group B and group C, control groups. The right tibial nerves of the rats were removed to create a 10 mm gap. The gaps in group A were bridged with the HHK artificial nerve, and other two groups with the nerve of its own or the homologous nerve （group C）. The blood samples were taken on 2, 8 and 16 weeks after operation to detect IgA, IgM, IgG, C3 ,C4, and T lymphocyte subgroup and T lymphocyte transformation. Results The immunological parameters between rats of groups A and B showed no statistical difference （F= 17.66- 671.34, q= 0.00- 2.09, P〉0.05）. The difference between groups C and A or B was statistically significant （q= 7.04-41.47,P〈0.01）. Conclusion Human-hair keratin artificial nerve, with its favourable histocompatibility, is an ideal graft material for peripheral nerve repair.