二苯乙烯苷对实验性动脉粥样硬化大鼠血脂和炎症因子的调节作用

Effect of tetrahydroxystilbene-glucoside on lipid and inflammatory factor levels of experimental atherosclerosis in rats

目的:探讨二苯乙烯苷(TSG)对大鼠动脉粥样硬化(AS)的预防作用。方法:SD大鼠60只,♂,随机分为6组:正常对照组(普通饲料组);模型组(高脂饲料);TSG低剂量组(30mg·kg-1·d-1);TSG中剂量组(60mg·kg-1·d-1);TSG高剂量组(120mg·kg-1·d-1);舒降之对照组(2mg·kg-1·d-1)。采用高脂饲料喂饲+VitD3复制大鼠AS模型。造模12周后,颈动脉取血,检测血清TG、TC、低密度脂蛋白-胆固醇(LDL-C)、高密度脂蛋白-胆固醇(HDL-C)、IL-6、TNF-α及C反应蛋白(CRP)。取血后分离主动脉,置于4%多聚甲醛液,作病理形态学观察。实验数据采用STATA7.0软件进行统计分析。结果:TSG120mg·kg-1·d-1和60mg·kg-1·d-1能显著降低血浆TG、TC、LDL-C、IL-6、TNF-α、CRP水平及升高HDL-C水平,并呈剂量依赖性。主动脉的苏丹Ⅳ和HE染色病理观察显示,TSG给药组主动脉内皮脂质沉积较模型组少见。结论:TSG对高脂饲料+VitD3诱导的大鼠AS形成具有预防作用,其机制可能与其调节血脂,抗炎作用有关。

AIM： To investigate the preventive effect of tetrahydroxystilbene-glucoside （TSG） on experimental atherosclerosis rats. METHODS： Sixty one male rats were randomly divided into six groups, normal control; model control; TSG low dose （ 30 mg·kg^-1·d^-1） ; TSG middle dose （ 60 mg·kg^-1·d^-1） ; TSG high dose （ 120 mg·kg^-1·d^-1）; simvastatin（2 mg·kg^-1·d^-1）. The AS model of rats was made by feeding high grease food and injecting VitD3 . All the rats were fed for 12 weeks, blood samples were drawn from carotid artery of rats, the levels of TC, TG, HDL-C, LDL-C , CRP, IL-6 and TNF-α in serum were measured with biochemical method. After blood samples were collected , the aorta samples were separated from the bodies,. then they were placed 4% paraformaldehydea and were through Sudan Ⅳ and HE staining. STATA7.0 software was used to evaluate the differences between groups. RESULTS： Data of the study demonstrated that the levels of TC, TG, LDL-C, TNF-α, IL-6 and CRP were decreased remarkably, the level of HDL-C was increased by TSG 60, 120 mg·kg^-1·d^-1 group in the high cholesterol-fed rats, which showed a dose-dependent effect. The result of Sudan Ⅳ and HE staining suggested that the lipid deposits in aortic endothelium in TSG group were less than those in model group. CONCLUSION： TSG has preventive effect on the experimental atherosclerosis among the high cholesterol-fed rats. The anti-atherogenic effect of TSG seems to be closely related to regulating plasma lipid profile, and inhibiting inflammation.