苄达赖氨酸对大鼠糖尿病肾病的防治作用(英文)

Protective effects of bendazac lysine against development of diabetic nephropathy

目的:在大鼠肾系膜细胞及整体大鼠上观察卞达赖氨酸(bendazac lysine,BDL)对糖尿病肾病(di-abetic nephropathy,DN)的防治作用。方法:大鼠肾系膜细胞分别在3种浓度的BDL中培养36h后,流式细胞仪测定细胞S期百分率;RT-PCR法测定转化生长因子(transforming growth factorβ1,TGF-β1)mR-NA水平;放免法测定细胞内Ⅳ型胶原和层粘蛋白含量;糖尿病模型大鼠灌胃3种剂量的BDL8周后,亲和层析法测定大鼠血清糖化血红蛋白。结果:高糖和H2O2可使S期细胞百分数大幅度降低,TGF-β1 mRNA相对含量、Ⅳ型胶原和层粘蛋白含量明显升高。低中高3个浓度的BDL皆可使S期细胞比例分别有不同程度的升高;使TGF-β1 mRNA相对含量、Ⅳ型胶原和层粘蛋白含量显著降低。中高剂量的BDL可显著降低大鼠血清糖化血红蛋白,并明显改善其生理和精神状态。结论:BDL升高大鼠肾系膜细胞的S期百分数,降低TGF-β1 mRNA、Ⅳ型胶原和层粘蛋白水平的作用与DN密切相关,是潜在的DN防治药物。

AIM： To investigate the protective effects of bendazac lysine （BDL） on diabetic nephropathy （DN） in vitro and in vivo experiments. METHODS： After rat mesangial cells were cultured in 3 concentrations of BDL for 36 h, the percentages of S phase of cells were determined by flowcytometry; the transforming growth factor β1 （TGF-β1） mRNA level was assayed by reverse transcription PCR; and two main components of extracellular matrix （ECM）, collagen Ⅳ and laminin, were determined by radioimmunoassay. Streptozotocin （STZ） induced diabetic rats were administered BDL at doses of 100, 200, 400 mg/kg for 8 weeks. The physical behavior and HbAlC levels of rats were observed. RESULTS： In the presence of high glucose and H2O2, the percentages of S phase of cells were lowered, and TGF-β1 mRNA level, collagen Ⅳ and laminin level were significantly increased. When compared with those in the high glucose group, the percentages of S phase of cells were signficantly raised, and the levels of TGF-β1 mRNA, collagen Ⅳ and laminin were statistically decreased. The physical behavior of high BDL treated rats restored to be vibrant, vigorous and weight gaining, and the HbAlC level was significantly reduced. CONCLUSION： BDL has the protective effects against damage caused by DN, and is a potential drug candidate worth further study in preventing and treating DN.