摘要
为了探讨急性白血病的免疫表型特征与诊断价值,各系相关抗体的敏感性和特异性,利用流式细胞仪进行三色免疫荧光标记技术检测白血病患者,根据抗体体积分系统进行分型.结果显示,三染色标记检测急性白血病患者中,急性淋巴细胞白血病（ALL）患者除均表达淋系抗原外,同时伴髓系抗原（CD13、CD33）的表达,其中cCD79a敏感性和特异性均较高.急性髓细胞性白血病（AML）患者除表达髓系抗原外,同时伴淋系抗原（CD19、CD7）表达,其MPO、CD117中特异性较高.结论：流式细胞仪三色免疫荧光直接标记法进行白血病免疫分型能够确诊一些特殊类型的白血病,对于急性白血病的诊断治疗和预后判断均有帮助.
To study the acute leukemia immunophenotype and its diagnosis value and the sensitivity,specificity of the lineage related antibedies, immunophenotyping in 102 patients with acute leukemia was performed by FCM analiysis. Theresults showed that patients with ALL expressed lymphocyteabtigent ,43.7% accompanied myeloid antient, the sensitivity and the specificity of cCD79a was higher, all patients with AML expressed myeloid antigent,34% accomanied lymphocyte antigent, the specificity of MPO and CD117 was higher. IN conclusion, leukemia immunophenotyping by three colour direct immfluore staining methods could define some paticular types of leukemia with an important value in diagnosis, treatment ang predicting prognosis of acute leukemia
