磺胺母核结构半抗原的合成与表征

Synthesis and Characterization of Sulfonamide Haptens with Mother Nuclear Structure

以4-乙酰氨基苯磺酰氯和含有氨基的羧酸或酯为原料,在90℃下,先以吡啶为溶剂,回流数小时后,加入NaOH为溶剂,蒸除吡啶,再回流数小时,最后用浓盐酸酸化得磺胺母核结构半抗原.合成的5种磺胺母核结构半抗原用1H NMR、FT-IR、ESI-MS进行了表征,为建立检测磺胺多残留的ELISA方法奠定了基础.

4-acetylsulfanilyl chloride and carboxylic acid or ester containing amino group as starting materials were heated under reflux at 90℃ in the pyridine. After several hours the sodium hydroxide was added, then removed the pyridine under vacuum and continued to reflux for several hours. Finally the pH of the mixture was adjusted with concentrated hydrochloride acid and obtained sulfonamide haptens with mother nuclear structure. Five sulfonamide haptens were synthesized and confirmed by ^1H NMR,FT-IR, ESI-MS, which were the basis of developing the ELISA methods for detecting sulfonamide muhiresidue.