性激素及其受体与肠易激综合征发病机制的试验研究

Sex hormones and their receptors in pathogenesis of IBS

目的检测肠易激综合征(irritable bowel syndrome,IBS)患者外周血中雌孕激素、睾酮激素水平及雌孕激素受体、睾酮激素受体在肠道内的分布、变化及临床表现,探讨性激素及其受体与IBS发病机制的可能关系。方法对符合罗马II标准的48例IBS患者及30例健康对照者的外周血中雌激素(estradiol,E2)、孕激素(progesterone,P)和睾酮激素(testosterone,T)的水平,采用放射免疫r计数器测定,并用自动软件分析。同时对68例IBS患者及60例健康对照者经结肠镜钳取回肠末端和降结肠黏膜组织,采用免疫组化法检测雌孕激素受体、睾酮激素受体蛋白表达。结果男性IBS患者外周血中睾酮激素含量下降(P<0.05)和肠黏膜睾酮激素受体高表达(P<0.05),女性IBS患者外周血中雌激素含量下降(P<0.05)和肠黏膜雌激素受体高表达(P<0.05)。结论IBS患者的发病可能与体内性激素水平紊乱有关,有利于进一步阐明性激素及其受体在IBS发病机制中的作用。

Objective To investigate the level of estrogen,progestogen and testosterone in peripheral blood, and observe the intestinal distribution, intestinal change and clinical significances of their receptors in patients with IBS. Methods Fourty eight patients with IBS were selected and identified by Rome Ⅱ diagnostic criteria and 30 healthy subjects were served as controls. The levels of sex hormone in peripheral blood were analyzed by radioimmunoassay, sixty eight patients with IBS and 60 healthy subjects were served as controls. Biopsies from 68 patients with IBS and 60 control persons were acquired by colonoscope. Number of positive cells for estrogen,progesterone and testosterone receptors in terminal ileum - cecum and decending colon was calculated after immunohistoehemieal staining. Results The blood testosterone in male IBS patients was lower than that in controls （P 〈 0.05）, while blood estradiol in female was lower than that in controls （P 〈 0.05）. Expressions of testosterone receptor in male IBS and estradiol receport in female IBS cases in intestinal mucosa were increased （P 〈 0. 05） compared with cotrols. Conclusion The results indicate that the patients with IBS might be associated with the level of sex hormone.